一株产β-葡萄糖苷酶烟曲霉菌株的鉴定及其产酶特性  

Identification of β-glucosidase producing Aspergillus fumigatus and its enzyme production characteristics

作　　者：刘德海[1] 马焕 解复红 权淑静 贾彬 王红云[1] 陈国参[1] 

机构地区：[1]河南省科学院生物研究所有限责任公司,河南郑州450008 [2]河南省工业酶工程技术研究中心,河南郑州450008

出　　处：《中国酿造》2015年第6期118-122,共5页China Brewing

基　　金：河南省中国科学院成果转移转化中心项目(2014308);河南省工程技术研究中心专项资金项目(132102213112)

摘　　要：从酿造大曲中分离筛选出一株产β-葡萄糖苷酶真菌菌株L1,根据对该菌株形态学观察和分子生物学18S rDNA分析,将其鉴定为烟曲霉（Aspergillus fumigates）,并对其所产β-葡萄糖苷酶酶学性质进行了研究.结果表明,该酶的最适反应温度为50℃,热稳定性较好,在50℃保温120 min仍能保持79.6％酶活性;最适反应pH值为4.0,在pH值3.0-5.0酶活性稳定.A β-glucosidase producing strain L1 was screened from Daqu. By physical characterization and 18S rDNA molecular identification, the strain L1 was identified as Aspergillus fumigatus. The enzymatic characteristics of β-glucosidase were studied as well, and the results showed that the optimum reaction temperature and pH were 50 ℃ and 4.0. After incubation at 50 ℃ for 120 min, it remained 79.6% of its activity. The enzyme was stable in the range ofpH 3.0-5.0.

关 键 词：烟曲霉 Β-葡萄糖苷酶 分离鉴定 酶学性质 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]