溶剂回流法提取石榴皮总多酚及抗氧化活性的研究  

Study on the Extraction of Total Polyphenols and Antioxidant Activity of Pomegranate Peel Bysolvent Refluxing Method

作　　者：王周[1] 张驰翔 蒲博[1] 朱奇奇 周金洋[1] 焦士蓉[1] 

机构地区：[1]西华大学生物工程学院,成都610039

出　　处：《食品工业》2015年第7期57-62,共6页The Food Industry

基　　金：教育部春晖计划项目(12205543);四川省教育厅自然科学重点项目(09ZA158);西华大学人才引进基金项目(R0820501)

摘　　要：采用溶剂回流提取方法,对石榴皮总多酚的提取工艺进行优化并测定其抗氧化活性。通过单因素和响应面优化设计确定了石榴皮多酚最佳提取工艺条件。同时研究了提取物对DPPH自由基、羟自由基、超氧阴离子自由基的清除作用以及对脂质过氧化物的抑制作用。结果表明,乙醇体积分数70%、提取温度89℃、料液比1︰26(g/mL)的条件下提取2.5 h,总多酚含量214.58 mg/g。提取物对DPPH自由基的半清除浓度EC50为0.014 mg/mL;对羟自由基的半清除浓度DC50为0.034 mg/mL;对超氧阴离子的半清除质量浓度HC50为3.82 mg/mL;对体外脂质过氧化的半抑制质量浓度IC50为0.019 mg/mL。Method for extracting by solvent reflux, optimization of extraction process on total polyphenols from pomegranate peel and its antioxidant activity was determined. Through the single factor and response surface optimization design to determine the optimum extraction conditions of Polyphenol from pomegranate peel. Also studied extracts on DPPH radicals, hydroxyl radicals, superoxide anion radical scavenging and inhibition of lipid peroxides. The results showed that in the conditions of 70% ethanol, extraction temperature 89 ~C, solid-liquid ratio 1 ： 26 （g/mL） and extracting time 2.5 h, the total polyphenol content was 214.58 mg/g. Extract on DPPH radical concentration EC50 was half cleared 0.014 mg/mL; half cleared to hydroxyl radical concentration DC50 was 0.034 mg/mL; half cleared on superoxide anion concentration HC50 was 3.82 mg/mL; on the half-inhibitory concentrations of lipid peroxides IC50 was 0.019 mg/mL.

关 键 词：石榴皮 总多酚 响应面分析法 抗氧化活性 

分 类 号：TS209[轻工技术与工程—食品科学]