纤维素酶提高马铃薯渣可溶性膳食纤维得率的工艺条件研究  

Study on production technology of raising the yield of soluble dietary fiber in potato residue with cellulase

作　　者：莎日娜[1] 韩育梅[1] 周霞[1] 杨钠[1] 

机构地区：[1]内蒙古农业大学食品科学与工程学院,内蒙古呼和浩特010018

出　　处：《食品工业科技》2015年第15期114-116,121,共4页Science and Technology of Food Industry

基　　金：内蒙古自然科学基金(2012MS1206)

摘　　要：以马铃薯干渣为原料,采用α-淀粉酶和蛋白酶提取膳食纤维后,用纤维素酶对其进行改性,研究酶添加量、p H、酶解温度和酶解时间对马铃薯渣可溶性膳食纤维得率的影响。在此基础上用正交实验优化酶反应的工艺条件。结果表明:酶添加量25 U/g,p H5,酶解温度45℃,酶解2.5 h为最佳反应条件。在此条件下可溶性膳食纤维得率为28.78%,而未用纤维素酶处理的得率为16.18%。通过AOAC 993.19酶-重量法测定马铃薯干渣中可溶性膳食纤维含量由7.01%提高至13.13%。Experiments were conducted to study the effect of cellulase amount, pH, enzymolysis time and temperature on the yield of soluble dietary fiber（SDF） which was modified by cellulase after the dieyary fiber pretreated by amylase and protease in the potato dry residue, on the basis of single factor experiment design orthogonakResults indicated that optimized conditions were pH5,45 ℃,25 U/g of cellulase and reaction time of 2. 5 h.The yield of SDF in potato residue has increased from 16.18% to 28.78%.The content of SDF in potato dry residue has increased from 7.01% to 13.13%.

关 键 词：马铃薯渣 可溶性膳食纤维 纤维素酶 工艺 

分 类 号：TS201.2[轻工技术与工程—食品科学]