酸性α-淀粉酶菌株的筛选及其发酵条件研究  

Screening of a Strain Producing Acid-stable α-Amylase and Optimization of Its Fermentation Condition

作　　者：屈建航[1] 尹伊[1] 焦国宝 丁长河[1] 张倩[1] 屈凌波[1] 刘仲敏 

机构地区：[1]河南工业大学生物工程学院,郑州450000 [2]河南仰韶生化工程有限公司,渑池472400

出　　处：《生物技术通报》2015年第7期188-192,共5页Biotechnology Bulletin

基　　金：国家科技支撑计划项目(2013AA102101);国家自然科学面上基金项目(31370147);河南省高校青年骨干教师资助计划(2013GGJS-077);河南省高校科技创新团队(15IRTSTHN019)

摘　　要：自淀粉厂周边土壤分离筛选到一株高效耐酸α-淀粉酶菌株SH3,初步鉴定为酵母菌,发酵粗酶p H范围3.8-8.0,最适作用p H5.0,该酶在80℃下仍有酶活,最适作用温度50℃。经单因素发酵条件的研究与优化,最适温度37℃,培养基初始p H5.0,可溶性淀粉作碳源,蛋白胨为氮源,200 m L装液量。正交试验确定最佳产酶条件为可溶性淀粉15 g/L、蛋白胨30 g/L、37℃、p H4.5,该条件下酶活力为96.8 U/mg。A strain SH3 producing acid-stable a-amylase was isolated from the soil nearing a starch factory. It was preliminarily identified as yeast. The pH range of crude enzyme reaction was 3.8-8.0 with the optimum of 5.0, and the activity of the enzyme was still available in the temperature of 80℃ with the optimum of 50℃. The optimization of single-factor fermentation revealed that the optimal condition for the enzyme production was 37℃, pH 5.0, soluble starch as carbon source, peptone as nitrogen source and 200 mL liquid volume. Orthogonal test showed that the optimal fermentation condition was as soluble starch of 15 g/L, peptone of 30 g/L, 37 ℃ and pH4. 5, under which the enzyme activity was 96.8 U/mg.

关 键 词：耐酸α-淀粉酶 菌株筛选 发酵条件 工艺优化 酵母菌 

分 类 号：TQ925[轻工技术与工程—发酵工程] Q93[生物学—微生物学]