高温高压提取甜菜废粕多糖的工艺及其抗氧化性研究  

The extraction conditions of polysaccharide from sugar beet pulp with high temperature and pressure and its antioxidant activity study

作　　者：赵亚红[1] 王文侠[1] 张显斌[1] 尚庆[1] 李长娟[1] 

机构地区：[1]齐齐哈尔大学食品与生物工程学院黑龙江省普通高校农产品加工重点实验室

出　　处：《食品与发酵工业》2015年第4期230-235,共6页Food and Fermentation Industries

摘　　要：甜菜废粕是甜菜制糖的副产物,是良好的食用、药用植物活性多糖来源。文中以甜菜废粕为原料,采用高温高压法对甜菜废粕多糖的提取工艺条件进行了研究,确定了最佳工艺条件为:固液比1∶30(g∶m L),温度115℃,时间4 h,在此条件下多糖得率为23.89%。采用高效凝胶渗透色谱法测定多糖的分子质量分布为337224.41、33 484.53和0.74 k Da。甜菜废粕多糖主要含有酸性多糖和中性多糖,同时还含有少量的阿魏酸和蛋白质。体外抗氧化实验表明,甜菜废粕粗多糖具有明显的抗氧化活性。Sugar beet-pulp is a by-product of sugar production and is a good source of edible,medical plant active polysaccharide. In this paper,polysaccharides extraction from beet pulp was studied by high temperature and pressure. The optimum technological conditions were： the ratio of the solid to liquid was 1∶ 30,the temperature was115 ℃ and the time was 4h. Under the above conditions,the yield of extracting polysaccharide was reached to23. 89%. The polysaccharide molecular weight distribution by high gel permeation chromatography and chemical composition were determined. The result shows the relative molecular of beet pulp polysaccharide was 337 224. 41 k Da,33 484. 53 k Da and 0. 74 k Da,respectively. Polysaccharide extracted from sugar beet pulp by high temperature and pressure was mainly composed of neutral sugar and acid sugar,and a small amount of ferulic acid and protein. Beetpulp polysaccharide had strong antioxidant activities.

关 键 词：高温高压法 甜菜废粕多糖 提取 抗氧化 

分 类 号：TS249[轻工技术与工程—制糖工程]