环介导等温扩增技术快速检测肉中沙门氏菌  

Rapid Detection of Salmonella in Raw Meat Samples by Loop-mediated Isothermal Amplification

作　　者：庞心怡[1] 满朝新[2] 赵玥明 周文琦[1] 闫天文[1] 柴云雷[1] 韩建春[1] 姜毓君[1,2] 

机构地区：[1]东北农业大学食品学院/乳品科学教育部重点实验室,哈尔滨150030 [2]东北农业大学国家乳业工程技术研究中心,哈尔滨150086

出　　处：《中国食物与营养》2015年第8期12-15,共4页Food and Nutrition in China

基　　金：国家科技支撑计划课题(项目编号:2012BAD28B02;2013BAD18B11;2012BAD29B07)

摘　　要：应用环介导等温扩增技术(loop-mediated isothermal amplification,LAMP)建立了一种快速、高效、灵敏的肉中沙门氏菌的检测方法。针对沙门氏菌的属特异性基因inv A设计2对引物,对人工污染肉样以及实际肉样分别进行检测。结果表明:所建立的LAMP反应能够特异性的检测沙门氏菌,对沙门氏菌纯菌的检测灵敏度为普通PCR的100倍,可达到9.8×100CFU/m L。LAMP检测人工污染沙门氏菌肉样的检测限为9.8×101CFU/m L。因此,本实验利用LAMP建立的沙门氏菌检测方法具有快速、灵敏、特异、操作简便的特点,具有广泛发展前景。A rapid,sensitive and efficient method to detect the Salmonella in meat samples was established by loop-mediated isothermal amplification（ LAMP）. Salmonella was detected in pure cultures and artificially inoculated meat samples with specifically designed primers targeting the inv A gene. Results showed that the LAMP assay could specifically detect 9. 8 × 10^0 CFU / m L Salmonella in pure cultures,100-fold sensitive than the traditional PCR. In the artificially inoculated meat samples,the detection limits could reach 9. 8 × 10^1 CFU / m L. In conclusion,the LAMP assay had advantages of rapidity,sensitivity,specificity and easy-to-operate and had great development potential.

关 键 词：环介导等温扩增 沙门氏菌 肉 

分 类 号：TS251.7[轻工技术与工程—农产品加工及贮藏工程]