响应面试验优化黑脉羊肚菌多酚纯化工艺及其抗氧化活性  

Optimization of Purification Process of Polyphenol from Morchella angusticepes Peck and Antioxidant Activity of the Extracted Polyphenol

作　　者：卢可可[1] 郑少杰[1] 吴素蕊[2] 廖霞[1] 明建[1,3] 

机构地区：[1]西南大学食品科学学院,重庆400715 [2]中华全国供销合作总社昆明食用菌研究所,云南昆明650223 [3]重庆市特色食品工程技术研究中心,重庆400715

出　　处：《食品科学》2015年第18期13-20,共8页Food Science

基　　金：国家自然科学基金面上项目(31471576);"十二五"国家科技支撑计划项目(2013BAD16B01)

摘　　要：以吸附率和解吸率为评价指标,研究9种大孔吸附树脂对黑脉羊肚菌多酚吸附及解吸性能,采用响应面法建立NKA-Ⅱ树脂纯化黑脉羊肚菌多酚的二次多项回归模型,对多酚的纯化工艺进行优化,并比较纯化前后多酚的抗氧化活性。结果表明:最佳纯化树脂为NKA-Ⅱ。吸附的最佳工艺条件为上样液质量浓度295.86μg/m L、上样流速1.90 m L/min、上样液p H 2.84,解吸的最佳工艺条件为乙醇体积分数78.56%、洗脱速率0.80 m L/min、洗脱剂p H 3.08;在此条件下吸附率可达98.69%,解吸率可达92.75%,纯化前后羊肚菌多酚纯度提高了2.94倍。黑脉羊肚菌多酚纯化前1,1-二苯基-2-三硝基苯肼自由基清除率、2,2’-联氮基-双-(3-乙基苯并噻唑啉-6-磺酸)二氨盐自由基清除率和还原力EC50值分别为1.48、0.015、2.35 mg/m L,纯化后分别为0.52、0.004、0.69 mg/m L,纯化后抗氧化活性明显增强。Nine macroporous resins were investigated with respect to their adsorption and desorption rates for the polyphenol extracted from Morchella angusticepes Peck in order to find the most appropriate one. Response surface methodology was utilized to establish a mathematical regression model for optimizing the purification of the crude extract by adsorption column chromatography with the selected resin. NKA-Ⅱ was found to be the most suitable resin for the purification of polyphenol. The optimum conditions for purification were obtained when the sample containing 295.86 μg/mL polyphenol at pH 2.84 was loaded onto the column at a flow rate of 1.90 mL/min, and then eluted with 78.56% aqueous ethanol acidified to pH 3.08 at a flow rate of 0.80 mL/min. The purification process yielded an adsorption rate of 98.69%, a desoprtion rate of 92.75% and a purification factor of approximately 4. The half maximal effective concentrations（EC50） for DPPH and ABTS radical scavenging capacities and reducing power of the crude extract were 1.48, 0.015 and 2.35 mg/mL and reduced to 0.52, 0.004 and 0.69 mg/mL after the purification, suggesting a significant increase in the antioxidant activities.

关 键 词：黑脉羊肚菌多酚 大孔吸附树脂 NKA-Ⅱ 响应面 抗氧化活性 

分 类 号：R151.2[医药卫生—营养与食品卫生学]