葛仙米多糖的单糖组成分析  

Analysis of Monosaccharide Composition of Polysaccharide from Nostoc sphaeroides Kützing

作　　者：莫开菊[1,2] 赵娜[1] 朱照武 田盼盼[1] 程超[1] 

机构地区：[1]湖北民族学院生物科学与技术学院,湖北恩施445000 [2]生物资源保护与利用湖北省重点实验室,湖北恩施445000

出　　处：《食品科学》2015年第18期89-92,共4页Food Science

基　　金：国家自然科学基金面上项目(31171670)

摘　　要：采用1-苯基-3-甲基-5-吡唑啉酮柱前衍生高效液相色谱(high performance liquid chromatography,HPLC)法及HPLC-质谱联用法测定葛仙米多糖的单糖组成,两种方法测定结果相互吻合。同时采用HPLC法测定了相关单糖的标准曲线,相关系数达到0.995以上,经计算,葛仙米多糖的单糖组成物质的量比甘露糖∶葡萄糖醛酸∶葡萄糖∶半乳糖∶阿拉伯糖为1∶1.12∶3.24∶1.72∶1.21。Two analytical methods, namely high performance liquid chromatography（HPLC） and HPLC-mass spectrometry（HPLC-MS） using precolumn derivatization with 1-phenyl-3-methyl-5-pyrazolone were established for analyzing the monosaccharide composition of polysaccharide from Nostoc sphaeroides Kützing. Consistent results were obtained using both methods. The standard curve developed using monosaccharide standards by HPLC exhibited a correlation coefficient of greater than 0.995. According to the standard curves, the polysaccharide in N. sphaeroides was composed of manose,glucuronic acid, glucose, galactose and arabinose at a molar ratio of 1：1.12：3.24：1.72：1.21.

关 键 词：葛仙米多糖 单糖 柱前衍生 高效液相色谱 质谱 

分 类 号：TS201.2[轻工技术与工程—食品科学]