金属膜在圆红冬孢酵母发酵液菌体分离中的应用  

Application of Metal Membrane for Separation of Rhodosporidium toruloides in the Fermentation Broth

作　　者：毕生雷 沈宏伟[2] 张成明[3,4] 金洪波[1] 吴娟[1] 鲁龙[1] 乔建援 刘钺[1] 

机构地区：[1]河南天冠企业集团有限公司车用生物燃料技术国家重点实验室,河南南阳473000 [2]中国科学院大连化学物理研究所,辽宁大连116023 [3]清华大学核能与新能源技术研究院,北京100084 [4]北京市生物燃料工程技术研究中心,北京100084

出　　处：《食品与发酵科技》2015年第5期1-6,共6页Food and Fermentation Science & Technology

摘　　要：针对产油微生物细胞采收难度大,能耗高的问题,采用金属超滤膜系统对圆红冬孢酵母发酵液进行浓缩和膜污染后通量恢复研究。结果表明在过滤时间2h、过滤温度35℃、p H 6.3、膜面流速5m/s、系统压力0.4MPa时,圆红冬孢酵母分离效果最佳,在此条件下进行过滤,平均膜通量达120L/m2·h,与之前相比提高了20-37%。膜污染后先使用添加1%Na OH和0.5%EDTA的碱溶液清洗,然后使用清水洗至中性,再使用添加0.5%HNO3的酸溶液清洗,能够使金属膜通量恢复率达到99%以上。以上结果表明,金属膜可以应用于圆红冬孢酵母发酵液的浓缩,能够简化操作步骤、提高生产效率。To resolve the problems of high difficulty and energy consumption in harvesting cells of oil-producing microorganism, a metal ultrafiltration membrane system was adapted to concentrate the fermentation broth with Rhodosporidium toruloides in this study. In addition, the flux recovery after membrane fouling was also investigated.Results showed that the optimal separation effect was obtained when filtration time was 2h, temperature 35℃, p H6.3, crossflow velocity 5m/s, operation pressure 0.4MPa. In this condition, the average permeate flux was 120L/m2·h which increased by 20-37% compared with previous experiment. When the fouled membrane was firstly cleaned by an alkaline solution（1% Na OH and 0.5% EDTA）, then by fresh water to neutral p H, and finally by an acidic solution with 0.5% HNO3, the permeate flux could recovery by exceeded 99%. In conclusion, the metal ultrafiltration membrane system could be applied to separate Rhodosporidium toruloides from the fermentation broth with simple operation and high productivity.

关 键 词：圆红冬孢酵母 发酵液 金属膜 膜通量 菌体分离 

分 类 号：TQ920.9[轻工技术与工程—发酵工程]