响应面法优化咖啡果皮可溶性膳食纤维提取工艺和功能特性研究  

Coffee Peel Soluble Dietary Fiber Extraction Technology via Response Surface Methodology Optimization and Functional Characteristics Analysis

作　　者：胡荣锁[1,2] 周晶[3] 董文江[1,2] 赵建平[1,2] 陆敏泉[1,2] 宗迎[1,2] 

机构地区：[1]中国热带农业科学院香料饮料研究所,海南万宁571533 [2]国家重要热带作物工程技术研究中心,海南万宁571533 [3]中国热带农业科学院热带生物技术研究所,海南海口571533

出　　处：《热带农业科学》2015年第9期66-72,共7页Chinese Journal of Tropical Agriculture

基　　金：中国热带农业科学院院本级基本科研业务费专项资金(No.1630052015043)

摘　　要：研究旨在应用酶法提取咖啡果皮中膳食纤维,为高品质膳食纤维提取及产业化开发应用提供参考。单因素及响应面优化纤维素酶法提取咖啡果皮中可溶性膳食纤维的工艺条件,并对其功能特性进行分析。结果表明:纤维素酶法提取的最优提取工艺为:超纯水(V)∶咖啡果皮粉(m)=100 m L∶6 g,纤维素酶活添加量为22 FPU/m L,酶解温度40℃,酶解时间2 h,在此工艺条件制备可溶性膳食纤维得率为9.72%;其持水力为(35.72±0.33)g/g,溶胀力为(94.85±0.23)m L/g,结合水力为(26.97±0.54)g/g。The aim of enzymatic method extract coffee peel soluble dietary fiber was to provide reference of high quality dietary fiber industrialization development. Single factor and response surface methodology as an approach to optimize the extraction technology of coffee peel soluble dietary fiber, while their functional characteristics were briefly analyzed. The results showed that： the optimum extraction process of cellulase extraction was as follows： ultrapure water（V） ∶coffee peel powder（m） =100 m L ∶6 g,cellulose enzyme activity adding amount was 22 FPU/m L, enzyme solution temperature was 40℃, enzyme solution time was 2 h, in this process condition the coffee peel soluble dietary fiber yield was 9.72 %. The functional characteristics was as follows： retention ability yield was（35.72±0.33） g/g, swelling force yield was（94.85±0.23） m L/g, bound moisture force yield was（26.97±0.54） g/g.

关 键 词：咖啡 果皮 可溶性膳食纤维 酶法提取 

分 类 号：TS273[轻工技术与工程—农产品加工及贮藏工程]