罗非鱼肉蛋白酶解液及其超滤液的抗氧化活性研究  

Study on antioxidant activity of Tilapia hydrolysate and its ultrafiltrate

作　　者：洪鹏志[1] 张鹏[1] 韩道财[1] 杨萍[1] 

机构地区：[1]广东海洋大学食品科技学院/广东普通高等学校水产品深加工重点实验室,广东湛江524088

出　　处：《广东农业科学》2015年第20期102-106,共5页Guangdong Agricultural Sciences

基　　金：广东海洋大学创新强校工程项目(GDOU2013050313;GDOU2014050203);省部产学研合作专项(2013B090800021)

摘　　要：通过研究罗非鱼肉蛋白酶解液及其5 ku超滤液的体外抗氧化活性,为罗非鱼深加工及高值化利用提供理论基础.主要采用分光光度法分别检测了酶解液和超滤液的还原力,对·OH、O2-·、DPPH·自由基的清除能力以及在亚油酸体系中抗氧化能力并对其抗氧化活性的差异性进行分析讨论.结果发现,当浓度为1.25～2.5 mg/mL时,超滤液的还原力、在亚油酸体系中抗氧化能力低于酶解液,当浓度为2.5～10.0 mg/mL时,超滤液活性强于酶解液;以IC50计算,超滤液对自由基清除能力均优于酶解液;超滤液中亮氨酸、苯丙氨酸、异亮氨酸、组氨酸和酪氨酸的含量均高于酶解液,其分子量小于3 ku与1 ku组分的比例也高于酶解液.罗非鱼肉蛋白酶解液经5 ku超滤处理后,其体外抗氧化活性增强.The vitro antioxidant activities of tilapia hydrolysate and its 5 ku ultra.filtrate were studied aimed at offering basic theory for tilapia processing and high-value utilization. The capacities on reducing power, · OH, O2- · , DPPH · free radical scavenging and its antioxidation in linoleic acid of hydrolyzate and uhrafihrate were investigated by spectrophotometry, as well as an in-depth analysis on its antioxidant activity variability was carried out. The results showed that when the concentration was 1.25-2.5 mg/mL, the reducing power and antioxidation in linoleic acid of ultrafihrate was lower than those of hydrolyzate, while the concentration was 2.5-10 mg/mL, the result was opposite. And the free radical scavenging of uhrafiltrate was better than that of hydrolyzate with half inhibitory concentration （ IC50 ） . The contents of Leu, Phe, Ile, His and Tyr in ultrafiltrate were higher than those in hydrolysate, in addition, the components which were lower than 3 ku or 1 ku, in ultrafihrate were higher than those in hydrolysate. The results indicated that compared with tilaDia hvdrolvsate, the antioxidant activity of 5 ku ultrafiltrate obviously increased.

关 键 词：酶解液 超滤液 自由基 抗氧化活性 氨基酸含量 

分 类 号：S917[农业科学—水产科学]