大米主料蛹虫草固体发酵生产纤溶酶的研究  

Production of Fibrinolytic Enzyme from Cordyceps militaris by Solid Culture Using Rice as a Major Material

作　　者：王玉[1] 刘晓兰[1] 

机构地区：[1]齐齐哈尔大学食品与生物工程学院,农产品加工黑龙江省普通高校重点实验室,齐齐哈尔161006

出　　处：《食品工业》2015年第12期22-26,共5页The Food Industry

摘　　要：以大米为主要原料,通过固态发酵的方法生产蛹虫草纤溶酶。运用单因素试验初步优化发酵的培养基组成及培养条件,用正交试验方法对蛹虫草固态发酵的无机盐添加量进行了细致探索。结果表明,试验范围内蛹虫草菌固态发酵生产纤溶酶的适合培养基主料为麸皮和大米,二者质量比为1︰1,培养基加水量75 g/100 mL,无机盐CaCl_2和(NH_4)_2SO_4的添加量分别为0.1 g/100 mL和2 g/100 mL,培养基初始pH自然,接种量25 g/100 mL,培养时间5 d,固体发酵物的纤溶酶浸提时间为4 h。优化条件下的固体发酵纤溶酶活力达167±1 U/mL。A f ibrinolytic component was produced from Cordyceps militaris by solid-state fermentation method using ric with wheat bran as main raw materials. The medium composition and culture conditions of the solid-state fermentation wer optimized by single factor experiment and orthogonal experiment method. The results showed that suitable solid medi components and culture condition were as followings： weight ratio of rice and wheat bran was 1︰1, water volume was 7g/100 mL, inorganic salt CaCl_2 and（NH_4）_2SO_4 was 0.1 g/100 mL and 2 g/100 mL, nature media pH, inoculation size wa25 g/100 m L, culture time was 5 d and extraction time was 4 h. Under the optimized fermentation conditions, the f ibrinolyti component activity was 167±1 U/mL.

关 键 词：大米 固态发酵 蛹虫草纤溶酶 酶活力 

分 类 号：TQ925[轻工技术与工程—发酵工程]