毕赤酵母工程菌发酵木聚糖酶条件的响应面优化  

Optimization of fermentation condition of Pichia pastoris for xylanase production by response analysis

作　　者：王丹丹[1,2] 周晨妍[1] 朱新术[1] 李同彪 

机构地区：[1]新乡医学院生命科学技术学院,河南新乡453000 [2]新乡医学院三全学院,河南新乡453000

出　　处：《食品工业科技》2016年第2期194-198,共5页Science and Technology of Food Industry

基　　金：河南省教育厅科学技术研究重点项目(13A180861);河南省高等学校青年骨干教师资助计划项目(2011GGJS-125);新乡医学院科研项目培育基金(2013ZD113);新乡医学院研究生科研创新支持计划项目资助(YJSCX201327Y)

摘　　要：通过单因素实验研究了诱导温度、种龄、p H、甲醇浓度以及诱导时间对毕赤酵母工程菌产木聚糖酶的影响。在此基础上进行响应面优化设计实验,并根据结果拟合小二乘二次项回归方程,探讨了各因素对木聚糖酶比酶活力的影响。确定了最优的发酵培养条件:种龄为31 h,诱导时间104 h,甲醇诱导浓度1%,发酵起始p H4.0,诱导温度为30℃,在此条件下对实验结果进行验证,得到木聚糖酶比酶活力为43526.3 U/mg。The influence of the temperature,inoculum time,p H,methanol concentration and induction time on the xylanase production of pichia recombinant bacteria were studied. The response surface optimazation was designed on the basis of the single factor experiment,and the small squares quadratic regression equation was fitted out according to the results to discuss the influence of the various factors on the xylanase enzyme activity. The optimum fermentation culture conditions were as follows：inoculum age 31 h,induction time 104 h,methanol concentration induced by 1%,fermentation starting p H4.0,induction temperature 30 ℃. On this condition,the result of the experiment for xylanase enzyme activity was 43526.3 U/mg.

关 键 词：毕赤酵母 木聚糖酶 发酵条件 响应面 

分 类 号：TS262.2[轻工技术与工程—发酵工程]