高效阴离子交换色谱法同时测定菊粉酶解产物中的单糖、双糖和低聚果糖  

Simultaneous Determination of Monosaccharides, Disaccharides, and Fructooligosaccharides in Inulin Hydrolysates by High Performance Anion Exchange Chromatography

作　　者：徐艳冰[1] 郑兆娟[2,3] 徐颖[1] 孙秀程 许茜茜[2] 欧阳嘉[2,3] 

机构地区：[1]南京林业大学林学院,江苏南京210037 [2]南京林业大学化学工程学院,江苏南京210037 [3]江苏省生物质绿色燃料与化学品重点实验室,江苏南京210037

出　　处：《食品科学》2016年第2期77-81,共5页Food Science

基　　金：国家自然科学基金青年科学基金项目(31200443;31300487);江苏省杰出青年基金项目(BK2012038);国家高技术研究发展计划(863计划)项目(2012AA022301);江苏省自然科学基金项目(BK20130970);江苏省重点研发计划项目(BF2015007)

摘　　要：建立了低聚果糖样品中的葡萄糖、果糖、蔗糖、蔗果三糖、蔗果四糖和蔗果五糖的高效阴离子交换色谱-脉冲安培法的定量分析方法。采用阴离子交换柱Carboa Pac^(TM) PA10(250 mm×2 mm)脉冲安培法进行检测,以氢氧化钠和醋酸钠为淋洗液进行梯度洗脱,柱温30℃,流速0.3 m L/min。结果表明,葡萄糖、果糖、蔗糖、蔗果三糖、蔗果四糖和蔗果五糖在质量浓度0.1~10 mg/L范围内的线性关系良好,相关系数r^2大于0.996 1,各组分的相对标准偏差均在2.69%~7.21%之间。将此方法应用于菊粉酶解后反应产物的检测,结果表明该方法快速简便且灵敏度高,能满足反应样品中低聚果糖的检测要求。An analytical method for the simultaneous determination of glucose, fructose, sucrose, kestose, nystose, and fructofuranosylnystose in fructooligosaccharide products was developed by using high performance anion exchange chromatography coupled with pulsed ampere detection（HPAEC-PAD）. The analysis was performed on a Carbo PacTM PA10 column（250 mm × 2 mm）by gradient elution using Na OH-Na Ac as the mobile phase. The column temperature was set at 30 ℃, and the flow rate was 0.3 m L/min. The results showed that the calibration curves developed for glucose, fructose, sucrose, kestose, nystose and fructofuranosylnystose had a good linear relationship within the concentration range of 0.1–10 mg/L（r^2 0.996 1）. The relative standard deviation for six repetitive determinations of each sugar was between 2.69% and 7.21%. This method is convenient, simple, and sensitive for the detection of fructooligosaccharide products from the enzymatic hydrolysis of inulin.

关 键 词：高效阴离子交换色谱 菊粉 低聚果糖 

分 类 号：TS245.9[轻工技术与工程—制糖工程]