粒毛盘菌多糖纯化、硫酸酯化修饰及抗氧化活性评价  

Purification and sulfated modification of a Lachnumpolysaccharide and evaluation of its antioxidant activity

作　　者：杜湛湛[1] 张嫱[1,2] 徐平[1] 王玉芬[1] 叶明[1] 

机构地区：[1]合肥工业大学生物与食品工程学院,安徽合肥230009 [2]北方民族大学生物科学与工程学院,宁夏银川750021

出　　处：《合肥工业大学学报（自然科学版）》2016年第1期134-139,144,共7页Journal of Hefei University of Technology：Natural Science

基　　金：国家自然科学基金资助项目(31470146)

摘　　要：文章以粒毛盘菌(Lachnum)为试验菌株,对其胞外多糖(LEP)进行纯化,并对获得的均一组分LEP-1进行硫酸酯化修饰,对修饰前多糖(LEP-1)和修饰后多糖(SLEP-1)进行了扫描电子显微镜、紫外和红外光谱表征,同时评价了两者的体外抗氧化活性。结果表明:LEP-1是由葡萄糖和甘露糖组成的杂多糖,分子量约为473kDa;SLEP-1的硫酸根取代度(DS)为1.97;SEM图显示修饰前后多糖表面形态由片状变化为网状;LEP-1的红外光谱图中有多糖的特征吸收峰,SLEP-1的红外光谱图除多糖的特征吸收峰外,在1 220cm-1和818cm-1处出现硫酸基团的特征吸收峰;LEP-1和SLEP-1对·OH、DPPH·和O2-·有很强的清除作用,SLEP-1的清除能力大于LEP-1,两者均呈现一定的剂量效应关系。因此,LEP-1和SLEP-1均有明显的抗氧化活性,且可作为天然抗氧化剂应用于食品和医药行业。A polysaccharide isolated from Lachnurn（LEP） was purified, and one of its faction（LEP-1） separated with Sephadex G-100 was sulfated, named as SLEP-1. The LEP-1 and SLEP-1 were charac- terized by scanning electron microscope（SEM）,UV and FT-IR spectra, and the antioxidant activities of them were also evaluated. The results showed that LEP-1 was a heteropolysaccharide, and consisted of glucose and mannose with the molecular weight of about 473 kDa. The degree of substitution （DS） of sulfated polysaccharide was 1.97. The SEM pictures showed that the surface morphology of LEP-1 was changed from laminar structure to reticulum after sulfation. The characteristic absorption peaks of polysaccharides appeared in the FT-IR spectrum of LEP-1, while the FT-IR spectrum of SLEP-1 also had the characteristic absorption peaks of polysaccharides, and the characteristic absorption peaks of sulfate groups of SLEP-1 appeared at 1 220 cm^-1 and 818 cm^-1 at the same time. The scavenging abilitie peaks of LEP-1 and SLEP-1 on · OH,DPPH · and O2^-· were strong, the scav- enging ability of SLEP-1 was stronger than LEP-1 and both of them showed a certain degree of doseresponse relationship. Therefore, both of LEP-1 and SLEP-1 had obvious antioxidant activities, and they could be applied as natural antioxidant in food and pharmaceutical industries.

关 键 词：粒毛盘菌 胞外多糖 修饰 抗氧化活性 

分 类 号：Q949.32[生物学—植物学] TS201.23[轻工技术与工程—食品科学]