常山胡柚降血脂成分提取工艺及其功能研究  

Study of Extraction for Hypolipidemic Components From the Peel and Pomace of Citrus Paradisi cv. Changshanhuyou and Its Application

作　　者：林敏[1] 任思婕 张真真[1] 唐仁龙[1] 吴界 潘家荣[1] 张永勇[1] 

机构地区：[1]中国计量学院,浙江杭州310018

出　　处：《核农学报》2015年第12期2343-2348,共6页Journal of Nuclear Agricultural Sciences

基　　金：浙江省"食品科学"重点学科资助项目(201302);浙江省第五届生命科学竞赛项目

摘　　要：为综合利用常山胡柚的果皮果渣,提高其经济价值,减少环境污染,以常山胡柚果皮果渣为原料,研究其所含黄酮类和柠檬苦素类物质的提取与纯化工艺,并对其降血脂辅助作用进行评价。结果表明：将原料烘干后磨成粉末,用95%乙醇提取5h,提取液经大孔树脂层析、蒸馏水去除多糖类物质、40%乙醇洗脱,洗脱液旋转蒸发蒸干,可得到纯化的黄酮类和柠檬苦素类物质,纯度为86%~99%,得率约为0.95%。胰脂肪酶活性抑制结果表明,制备的活性成分具有较强的降血脂作用。在此基础上,初步制成胶囊状保健品。本研究为常山胡柚的综合利用提供了新的途径。The comprehensive use of of peel and pomace of Citrus Paradisi cv. Changshanhuyou in China is of importance considering the increase of economy benefit and the decrease of environmental pollution,the objective of this experiment is to study the techniques for extraction,separation and purification of the active components,flavonoids and limonin,in the peel and pomace of Citrus Paradisi cv. Changshanhuyou,and to preliminarily assess their hypolipidemic effects.Optimized techniques for extraction and purification of flavonoids and limonin from the peel and pomace of Citrus Paradisi cv. Changshanhuyou was established,i. e. the dried peel and pomace was ground and extracted with 95%ethylthanol for 5 hours,then the extract passed through a column of macroporous resin,which was then washed with40% ethanol. Finally the washing solution was dried with rotary evaporator to obtain the active products. The extraction rate was about 0. 95% and the purity was about 86% to 99%. From the result of experiment on inhibition of the activity of pancreatic lipase,it was shown that the purified products presented obvious hypolipidemic effects. Finally,some small specimens with capsule shape had been prepared. The experiment would be providing a pathway for utilization of peel and pomace of Citrus Paradisi cv. Changshanhuyou,

关 键 词：常山胡柚 黄酮类物质 柠檬苦素类物质 降脂 

分 类 号：R151[医药卫生—营养与食品卫生学] TQ464[医药卫生—公共卫生与预防医学]