纤维素酶法提取玉米麸皮多糖的工艺条件优化  

Optimization of extraction of polysaccharides from corn fiber by celluclast

作　　者：王文侠[1] 尚庆[1] 李馥邑 张显斌[1] 宋波[1] 

机构地区：[1]齐齐哈尔大学食品与生物工程学院,黑龙江省普通高校农产品加工重点实验室,齐齐哈尔161006

出　　处：《中国食品添加剂》2016年第3期82-91,共10页China Food Additives

基　　金：黑龙江省自然科学基金项目(B201115)

摘　　要：玉米麸皮是玉米湿法加工的主要副产物,是良好的食用、药用植物多糖来源。以未脱脂玉米麸皮为原料,将经酶法去除淀粉和蛋白质后的皮渣采用复合纤维素酶法浸提玉米麸皮多糖(ECP),通过响应面法优化确定了酶法提取最佳工艺条件为:木聚糖酶∶复合纤维素酶=1∶1,加酶量1.5%(对底物),p H 4.1,底物浓度5g/100m L,酶解时间5h,酶解温度55℃,多糖得率为27.25%。玉米麸皮多糖主要组分的相对分子量为1720、911KDa,含有一定量的蛋白质和酸性糖以及微量的阿魏酸。Corn fiber is the main byproduct of corn wet milling processing and a good polysaccharide source in food and medicine. In this paper,the Cellulase extraction of corn fiber polysaccharides（ECP）were prepared from the corn fiber residue,and starch and protein were all removed. By the response surface experiment design,the optimum conditions to extract corn fiber polysaccharides were ：ratio of Shearzyme ：Celluclast,1 ∶ 1,enzyme dose1.5%（base substrate）,p H 4.1,substrate concentration 5.0g/100 m L,time 5h,temperature 55℃. The yield at the optimal extraction condition was 27.25%.The average molecular weight of main components ECP was 1720,911 KDa. Fractions of ECP contains a small amount of protein,acidic polysaeeharides,and trace of ferulic acid.

关 键 词：玉米麸皮 多糖 纤维素酶 提取 工艺优化 

分 类 号：TS210.9[轻工技术与工程—粮食、油脂及植物蛋白工程] Q629.12[轻工技术与工程—食品科学与工程]