响应面法优化嗜热链球菌产胞外多糖的发酵条件  

Optimization of fermentation conditions for extracellular polysaccharide production by streptococcus thermophilus with response surface methodology

作　　者：陈亚[1] 梁琪[1] 杨敏[1,2] 张卫兵[1] 张炎[1] 

机构地区：[1]甘肃农业大学食品科学与工程学院,甘肃兰州730070 [2]甘肃农业大学理工学院,甘肃兰州730070

出　　处：《食品工业科技》2016年第9期195-201,共7页Science and Technology of Food Industry

基　　金：兰州市研政产合作项目(2012-2-87);兰州市科技创新人才团队培育计划项目(2011-1-144)

摘　　要：本实验采用响应面法(RSM)对影响嗜热链球菌(Q4)产胞外多糖(EPS)的发酵条件进行了优化。以EPS含量为指标,通过单因素实验初步得到Q4产EPS的发酵条件;采用Placket-Burman(PB)设计法从以上单因素中筛选出显著因素,对显著因素进行Box-Behnken(BB)中心组合实验设计优化,建立EPS含量与各影响因素的回归方程,获得Q4产胞外多糖的最佳发酵条件为:初始p H6.8、发酵时间33 h、发酵温度41℃。验证实验结果表明,在此条件下胞外多糖的产量可达268.42 mg/L,比优化前的EPS含量(89.43 mg/L)提高了3倍。The quantitative effects of fermentation conditions on the yield of Streptococcus thermophilus（ Q4）extracellular polysaccharide were optimized using response surface methodology（ RSM）.The EPS content as an index,fermentation conditions of EPS produced by Q4 was preliminary obtained by single factor experiment.Then significant factors were screened from the above the single factors according to the Placket- Burman（ PB） design method and were optimized using Box- Behnken design.And the EPS content as the response values,obtaining the response surface figure,the optimal fermentation conditions on the yield of（ Q4） extracellular polysaccharide were determined： initial p H6.8,fermentation time 33 h and fermentation temperature 41 ℃.Under the optimal conditions the corresponding response value for extracellular polysaccharide production was 268.42 mg / L,compared with the previous EPS content（ 89.43 mg / L）,which was increased 3 times.

关 键 词：响应面法 嗜热链球菌 胞外多糖 培养条件 

分 类 号：TS201.3[轻工技术与工程—食品科学]