能力验证样品中沙门氏菌的分离与鉴定  

Isolation and Identification of Salmonella in the Proficiency Testing Sample

作　　者：王志伟[1] 徐琼[1] 陈欣钦[1] 曲勤凤[1] 

机构地区：[1]上海市质量监督检验技术研究院,上海200233

出　　处：《食品研究与开发》2016年第7期161-163,共3页Food Research and Development

摘　　要：通过参加能力验证,提高检测技能,验证实验室的检测能力。依据GB4789.4-2010《食品安全国家标准食品微生物学检验沙门氏菌检验》方法对代码为A414的样品进行沙门氏菌的检测鉴定。按照标准操作,使用TTB和SC两种增菌液,BS、XLD和沙门显色平板结合使用,通过API20E、VITEK并结合血清学试验综合判定,从能力验证样品中准确鉴定出肠道沙门氏菌亚种Ⅲa,即亚利桑那亚种,同时从样品中还检测出阴沟肠杆菌和奇异变形杆菌两种干扰菌。To strengthen laboratory detection capability and validate the capability of microorganism detection by ability testing. Testing Salmonella to sample A414 according to national standard. Identification of Salmonella isolates must be operated according to standard, using TTB and SC two kinds of enrichment broth, BS,XLD and the chromogenic medium three kinds of selective tablets. Comprehensive determination of the API20 E,VITEK identification and serological testing. Salmonella Ⅲ a was accurately identified from the sample. At the same time, Enterobacter cloacae and Proteus mirabilis were identified from the same sample.

关 键 词：能力验证 沙门氏菌 分离 鉴定 

分 类 号：TS207.4[轻工技术与工程—食品科学]