紫薯花青素的提取及其在VC含量测定中的应用  

Anthocyanins from Purple Sweet Potato(Ipomoea batatas) and Its Application in VC Quantification

作　　者：郭彩华[1] 卢珍华[1] 伍菱[1] 陈昭华[1] 陈庆绸[1] 熊何健[1] 

机构地区：[1]集美大学食品与生物工程学院,福建厦门361021

出　　处：《食品科学》2016年第9期134-138,共5页Food Science

基　　金：福建省自然科学基金资助项目(2013J01134)

摘　　要：从紫薯中提取花青素,该花青素在530 nm波长处有特征吸收峰,分子质量范围在850～1 020 u之间。利用紫薯花青素替代GB/T 6195—1986《水果、蔬菜维生素C含量测定法（2,6-二氯靛酚测定法）》测定夏橙和西红柿中VC含量。结果表明：夏橙和西红柿中VC的含量分别为（25.33±0.34）、（20.41±0.32）mg/100 g;紫薯花青素法的加标回收率为95.4%～98.6%,相对标准偏差（relative standard deviation,RSD）为1.18%～1.22%;2,6-二氯靛酚法的加标回收率为96.5%～98.7%,RSD为0.86%～0.88%。建立的利用紫薯花青素替代GB/T 6195—1986中2,6-二氯靛酚测定水果、蔬菜中VC含量的方法具有安全、廉价的特点,其准确度与2,6-二氯靛酚法滴定法相当。Anthocyanins were extracted from purple sweet potato（Ipomoea batatas） using an environmentally friendly process. The anthocyanins exhibited characteristic absorption peak at 530 nm, having a molecular weight in the range of 850-1 020 u. The content of vitamin C in Valencia orange and tomato was determined by a new titration method using the anthocyanins extracted from purple sweet potato in place of 2,6-dichloroindophenol as described in the Chinese national standard GB/T 6195-1986. The results showed that vitamin C was（25.33 ± 0.34） mg/100 g in Valencia orange and（20.41 ±0.32） mg/100 g in tomato. The spiked recoveries of VC with purple sweet potato anthocyanins ranged from 95.4% to 98.6%, with relative standard deviation（RSD） of 1.18%-1.22%, while those with 2,6-dichloroindophenol titration were between 96.5% and 98.7% with RSD of 0.86%-0.88%. The anthocyanin titration method was safe and low-cost with an accuracy equivalent to that of 2,6-dichloro-indophenol titration.

关 键 词：紫薯 花青素 GB/T 6195—1986 2 6-二氯靛酚 VC 

分 类 号：TS255[轻工技术与工程—农产品加工及贮藏工程]