长白山核桃楸分离蛋白缓解小鼠体力疲劳的作用  

Effect of Juglans mandshurica Maxim Kernel Protein Isolate on Alleviating Physical Fatigue in Mice

作　　者：崔玲玉 闵伟红[1] 刘威[1] 毛晶[1] 苗欣宇[1] 刘景圣[1] 

机构地区：[1]小麦和玉米深加工国家工程实验室,吉林农业大学食品科学与工程学院,吉林长春130118

出　　处：《食品科学》2016年第9期203-207,共5页Food Science

基　　金：国家高技术研究发展计划(863计划)项目(2013AA102206)

摘　　要：为探究长白山核桃楸分离蛋白（Juglans mandshurica Maxim kernel protein isolate,JMKP）缓解体力疲劳的作用,通过小鼠负重游泳实验,以游泳时间为疲劳指标,并测定血乳酸（blood lactic acid,BLA）、肝糖原（hepatic glycogen,GLU）和丙二醛（malondialdehyde,MDA）含量及谷胱甘肽过氧化物酶（glutathione peroxidase,GSH-Px）、过氧化氢酶（catalase,CAT）和超氧化物歧化酶（superoxide dismutase,SOD）活力等生化指标。结果表明：2周后,JMKP高剂量组小鼠的游泳时间比对照组增加了38.66%,4周后,JMKP低、中、高剂量组小鼠的游泳时间比对照组分别延长了24.10%、42.17%和64.46%;2周后,JMKP高剂量组小鼠的血乳酸含量与对照组相比下降了21.44%,4周后,JMKP中、高剂量组小鼠的血乳酸含量与对照组相比分别下降了13.02%和23.48%;2周和4周后,JMKP中、高剂量组小鼠的肝糖原含量与对照组相比分别升高了15.41%～16.29%和18.35%～20.81%;2周和4周后,JMKP高剂量组小鼠血清中的MDA含量与对照组相比分别下降了20.41%和28.59%;JMKP能够提高小鼠体内GSH-Px、CAT和SOD活力,特别是在灌胃高剂量的JMKP 4周后,与对照组相比均有极显著差异（P〈0.01）。实验结果表明JMKP具有显著缓解小鼠疲劳的作用。To explore the anti-fatigue effect of Juglans mandshurica Maxim kernel protein isolate（JMKP）, weight-loaded swimming test of mice was carried out and exhaustive swimming time as an indicator of fatigue, blood lactate（BLA） content, hepatic glycogen（HG） content, malondialdehyde（MDA） content, and the activities of glutathione peroxidase（GSH-Px）, catalase（CAT） and superoxide dismutase（SOD） were determined. The results showed the exhaustive swimming time of mice gavaged for 2 weeks with JMKP at high dose increased by 38.66% compared with controls, and 4-week administration at low, medium and high doses resulted in a 24.10%, 42.17% and 64.46% compared with controls, respectively. The BLA content of mice in the high-dose JMKP group administered for 2 weeks decreased by 21.44%, and that of mice from the medium-dose and high-dose JMKP groups administered for 4 weeks decrease by 13.02% and 23.48%, respectively, compared with controls. The GLU content of mice from the medium-dose and high-dose JMKP groups administered for 2 and 4 weeks increased by 15.41%–16.29% and 18.35%–20.81%, respectively compared with controls. High-dose administration of JMKP for 2 and 4 weeks led to a 20.41% and 28.59% in serum MDA content, respectively. The levels of GSH-Px, CAT and SOD activity could be significantly improved by JMKP administration, especially for 4-week high-dose JMKP compared with controls（P〈0.01）. Therefore, JMKP could evidently alleviate physical fatigue in mice.

关 键 词：长白山核桃楸 分离蛋白 体内实验 缓解疲劳 抗氧化性 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]