酶法合成中合成酶的应用研究  

Research on synthase application in enzymatic synthesis

作　　者：陈华磊[1,2] 侯殿志 于玥[1,2] 相萍萍 朱延光[1,2] 蓝尉冰[1,2,3] 谢政[1,2] 姚晓麦[1,2] 陈山[1,2] 

机构地区：[1]广西大学糖业工程技术研究中心,南宁530004 [2]糖业及综合利用教育部工程研究中心,南宁530004 [3]钦州学院,钦州535011

出　　处：《中国食品添加剂》2016年第6期114-124,共11页China Food Additives

基　　金：国家自然科学基金项目(21264003);2013年广西自然科学基金项目(2013GXNSFAA019036)

摘　　要：酶法合成因其高效无污染、专一性强、杂质含量低、可连续化生产及产品分子量易控制等优点,逐渐成为现在制备生物大分子化合物研究的热点。尤其是合成酶的应用,为生物大分子化合物的酶法合成提供了一种更加有效便捷的方式。但是,目前酶法合成的相关研究尚处于探索阶段,合成酶的研究报道也较少。探索获得高活性、高稳定性的合成酶,是酶工程技术制备生物大分子的关键。该文就合成酶的种类及应用、作用机制、以及催化性能提升等方面的国内外研究现状进行了综述,对其应用前景及未来的研究方向进行了探讨。Enzymatic synthesis has become a hot research topic in the synthesis of high molecular weight compounds due to the advantages of efficiency of the synthesis,pollution-free,high specificity,low impurity content,continuous producing and easily control of molecular weight.This study provides a more efficient and convenient way for the synthesis of biological macromolecules by enzymatic synthesis. The research of enzymatic synthesis is still in the exploratory stage and the reportsare few. To explore high activity and high stability of the synapses is the key in the enzyme engineeringof preparation of biological macromolecules. In this paper,the types and application of enzymes,the mechanism of action,and the improvement of the catalytic performance,as well as other aspects of domestic and foreign research status are reviewed,and its application prospects and future research directions are discussed.

关 键 词：酶法合成 合成酶 作用机制 催化性能 

分 类 号：Q559[生物学—生物化学]