番茄红素在大鼠肝脏中的几何异构体组成  

Geometric Isomer Composition of Lycopene from Rat Liver

作　　者：惠伯棣[1] 冯润月 宫平[1] 

机构地区：[1]北京联合大学应用文理学院食品科学系,北京100191

出　　处：《食品科学》2016年第13期189-193,共5页Food Science

基　　金：北京市教委科技发展计划重点项目(KZ20051147020)

摘　　要：目的:测定大鼠肝脏组织中番茄红素的含量及番茄红素几何异构体(顺/反异构体)的组成,为番茄红素在体内的代谢及生物学功能研究提供参考。方法:选用大鼠为动物模型,用番茄提取物配制灌胃液。一次灌胃8 h后取大鼠肝脏组织,提取其中的番茄红素。应用C30-高效液相色谱-光电二极管阵列(C30-high performance liquid chromatography-photodiode array detection,C30-HPLC-PDA)法测定提取物中番茄红素的总量和几何异构体组成。结果:大鼠肝脏中总番茄红素含量为92.95μg/g(以鲜质量计)。与灌胃液中的番茄红素几何异构体组成比较,大鼠肝脏组织中存在更多数量和种类的顺式异构体,包括13Z-番茄红素、11Z-番茄红素、9Z-番茄红素和5Z-番茄红素异构体。结论:这一现象对研究番茄红素在肝脏内的贮存、靶向调动和生物学功能具有重要意义。Total lycopene amount and lycopene geometric isomer composition of rat liver were determined in this study, aiming to lay a foundation for in vivo studies on lycopene metabolism and biological function. In the experiment, male rats were administrated with tomato extract at a single dose. After 8 h, liver tissues were collected for the extraction of lycopene. The total amount and geometric isomer composition of lycopene were determined by C30-high performance liquid chromatography with photodiode array detection（C30-HPLC-PDA）. Results indicated that total amount of lycopene from liver tissues was 92.95 μg/g fresh weight. In comparison with tomato extract, the composition and amount of lycopene Z-isomers（including 13Z-, 11Z-, 9Z- and 5Z-isomer） were increased in rat liver tissues. This phenomenon is important for investigating the storage, targeting transfer and biological function of lycopene in liver.

关 键 词：番茄红素 几何异构体 肝脏 代谢 

分 类 号：TS201.2[轻工技术与工程—食品科学]