LC-MS/MS法测定食用油中的辣椒素和二氢辣椒素  

Determination of capsaicin and dihydrocapsaicin in edible oil by LC-MS/MS

作　　者：梁桂娟[1] 寻思颖[1] 张琼[1] 李红洲[1] 

机构地区：[1]贵州省产品质量监督检验院,贵州贵阳550016

出　　处：《中国酿造》2016年第7期175-179,共5页China Brewing

基　　金：贵州辣椒技术标准体系(黔卫计办函[2015]94号)

摘　　要：建立了用氨基固相萃取小柱净化提取液，液相色谱串联质谱（LC-MS／MS）法测定食用油中辣椒素和二氢辣椒素含量的检测方法。结果表明，辣椒素在0--100ng／mL范围内线性关系良好（R2=0．9986），平均回收率在70．6％-90．5％，相对标准偏差（RSD，n=3）在3．1％～8．9％，检出限为0．03ixg／kg,二氢辣椒素在0～20ng／mL范围内线性关系良好（R2=0．9988），平均回收率在71．5％～100．7％，相对标准偏差（RSD，n=3）在6．O％～13．2％，检出限为0．006p,g／kg。该方法简便快速、准确可靠，是鉴别地沟油和测定食用油中辣椒素类物质的有效方法。A method was established for the determination of capsaicin and dihydrocapsaicin in edible oil by solid phase extraction and LC-MS/MS. The results indicated that the capsaicin showed good linear relationship in the range of 0-100 ng/ml （R2=0.998 6）, the average recovery was 70.6%-90.5%, and relative standard deviation （RSD, n=3） was 3.1%-8.9%, and the detection limit was 0.03 μg/kg. The dihydrocapsaicin showed good linear relation- ship in the range of 0-20 ng/ml （R2=0.998 8）, the average recovery was 71.5%-100.7%, relative standard deviation （RSD, n=3） was 6.0%-13.2%, and the detection limit was 0.006 Ixg/kg. The method was simple, rapid, accurate and reliable, and it was an effective method for the identification of waste oil and determination of capsaicin in edible oil.

关 键 词：固相萃取 液相色谱串联质谱 食用油 辣椒素 二氢辣椒素 

分 类 号：O657.72[理学—分析化学]