PMP-HPLC-MS^n法分析弱酸降解鲍鱼性腺多糖产生的寡糖  

Analysis of Oligosaccharides Released from Weak Acid Hydrolysis of Abalone Gonad Polysaccharide Using PMP-HPLC-MS^n

作　　者：鲁姣姣[1,2,3] 艾春青[1,2,3] 温成荣[1,2,3] 徐鑫[1] 张豹[1] 宋爽[1,2,3] 

机构地区：[1]大连工业大学食品学院,辽宁大连116034 [2]国家海洋食品工程技术研究中心,辽宁大连116034 [3]海洋活性多糖开发应用技术国家地方联合工程实验室,辽宁大连116034

出　　处：《食品科学》2016年第16期112-116,共5页Food Science

基　　金：国家自然科学基金青年科学基金项目(31301431;31501439);辽宁省农业领域青年科技创新人才培养计划项目(2014002);辽宁省教育厅一般项目(L2014222)

摘　　要：为考察鲍鱼性腺硫酸多糖(abalone gonad sulfated polysaccharide,AGSP)在弱酸降解过程中产生的寡糖,对不同酸浓度的降解产物1-苯基-3-甲基-5-吡唑啉酮(1-phenyl-3-methyl-5-pyrazolone,PMP)衍生化后,进行高效液相色谱-质谱(high performance liquid chromatography-mass spectrometry,HPLC-MSn)法检测。在AGSP的弱酸降解产物中发现了2种二糖(disaccharides,DS1、DS2)和1种四糖(tetrasaccharide,TS)。2种二糖均是由己糖醛酸通过糖苷键连于己糖,其中DS2的结构确定为β-Glc A(1→2)-Man,而TS是由2个DS2连接而成。在100℃加热1 h的条件下,在0.1~2.0 mol/L的三氟乙酸浓度范围内,这3种寡糖的产率都随着酸浓度的增加呈升高趋势。In order to investigate oligosaccharides released from abalone gonad sulfated polysaccharide （AGSP） throughweak acid hydrolysis, hydrolysates obtained at different acid concentrations were labeled with 1-phenyl-3-methyl-5-pyrazolone （PMP）, and then analyzed by high performance liquid chromatograph-mass spectrometry （HPLC-MSn）. Twodisaccharides （DS1 and DS2） and one tetrasaccharide （TS） were found in the AGSP hydrolysates. The two disaccharideswere composed of a hexuronic acid linked to a hexose through a glucosidic bond. DS2 was deduced as β-GlcA（1→2）-Man,and TS was proposed as a dimer of DS2. In hydrolysis reaction with 0.1–2.0 mol/L trifluoroacetic acid at 100 ℃ for 1 h, theyields of the three oligosaccharides all increased with increasing acid concentration.

关 键 词：鲍鱼 多糖 酸降解 质谱 寡糖 

分 类 号：R931.77[医药卫生—生药学]