超声解聚对大粒车前子多糖流变性质、溶液构象及活性的影响  

Effects of ultrasonic depolymerization on the rheological property,solution conformation and activities of polysaccharides isolated from the seeds of plantago asiatica L.

作　　者：夏强[1] 黄丹菲[1] 余强[1] 聂少平[1] 谢明勇[1] 

机构地区：[1]南昌大学食品科学与技术国家重点实验室,江西南昌330047

出　　处：《食品工业科技》2016年第17期80-85,共6页Science and Technology of Food Industry

基　　金：国家自然科学基金重点资助项目(31130041);国家自然科学基金资助项目(31260364);江西省教育厅青年科学基金项目(GJJ11050);食品科学与技术国家重点实验室青年骨干研究基金项目(SKLF-QN-201107)

摘　　要：研究超声波对大粒车前子多糖溶液流变性质、分子量及分子量分布、溶液构象、抗氧化活性、体外免疫活性功能的影响。用流变仪检测其静态动态流变性质。通过光散射凝胶色谱联用检测多糖分子量及溶液构象。使用红外光谱对多糖结构进行表征。通过体外抗氧化模型:DPPH自由基清除,铁离子还原,氧自由基清除评价抗氧化能力。通过小鼠巨噬细胞RAW264.7增殖,中性红吞噬及NO分泌评价体外免疫活性。结果表明超声之后大粒车前子多糖表观粘度急剧下降,流体性质从假塑性流体变成牛顿性流体;弹性模量G'与粘性模量G″均发生下降,凝胶性质减弱;分子量下降,分子量分布先变宽后变窄;结构参数ρ下降,Mark-Houwinkα值增大,溶液构象从高支化结构向无归线团转变;特性粘度[η]下降,多糖在溶液中的构象更紧凑;主要吸收峰没变,多糖的主要官能团并未改变;DPPH自由基清除能力和铁离子还原能力显著提高,氧自由基清除能力变化不显著;促进巨噬细胞吞噬、增殖、分泌NO的能力均显著提高。To study the ultrasonic degradation＇ s effects on rheological properties, molecular weight and molecular weight distribution, conformation in solution, antioxidant activity and in vitro immune of polysaccharide isolated from the seeds of Plantago asiatica L. （ PLCP）, rheometer was used to detect static and dynamic rheological property of these treated samples,GPC/SEC-MALS system was used to detect molecular weight and solution conformation, infrared spectroscopy was used to characterize the structure of the polysaccharide. In vitro antioxidant activities were evaluated by DPPH radicals scavenging capacity, ferric ion reducing antioxidant capacity, and oxygen radical absorbance capacity. The in vitro immunoregulatory activities was detected by macrophage RAW264.？ proliferation assay, neutral red phagocytosis test and NO production test.Ultrasonic degradation＇ s effects on PLCP were as follow：the apparent viscosity was sharply declined, rheological property was changed from pseudoplastic fluid into Newtonian fluid.The elastic modulus G＇ and the viscous modulus G＂ were reduced, gel properties were decreased.Molecular weight was degraded,the molecular weight distribution was narrowed after the first widen. Structural parameters p was decreased, Mark-Houwink a value was increased.Solution conformation changed from highly branched structure into a random coil. The intrinsic viscosity [η] was decreased.The solution had more compact conformation.The main infrared absorption peak did not changed,the main polysaccharide functional groups did not changed.DPPH radical scavenging and ferric reducing ability was increased,oxygen free radical scavenging capacity did not changed significantly, ability for promoting macrophage phagocytosis, proliferation, secrete NO were significantly increased.

关 键 词：多糖 超声波降解 流变性质 溶液构象 抗氧化 免疫调节 

分 类 号：TS201.2[轻工技术与工程—食品科学]