大孔树脂纯化叶多酚及其对胰淀粉酶的抑制作用  

Study on polyphenol purification by macroporous resin and inhibition activity to pancreatic amylase of polyphenol from Docyniaindica (Wall.) Dcne leaves

作　　者：陈江[1] 张晓喻[1] 刘刚[1] 王战国[2] 刘海霞[1] 

机构地区：[1]四川师范大学生命科学学院,四川成都610100 [2]成都大学医学院(护理学院),四川成都610100

出　　处：《食品工业科技》2016年第18期143-148,共6页Science and Technology of Food Industry

基　　金：四川省科技厅科技支撑项目(2016SZ0026);四川师范大学校级重点培育项目(13ZDL11);四川省教育厅项目(14ZA0025);攀枝花市科技局项目(2014CY-S-8);西昌市教科和科学计划局2014年度科技项目;四川师范大学大精基金项目(DJ2013-02;DJ2014-13;DJ2015-04)

摘　　要：研究栘叶多酚的大孔吸附树脂纯化工艺,并考察其纯化前后对胰淀粉酶的抑制作用。通过静态吸附解析实验筛选出吸附性能和解吸性能良好的D-101型大孔吸附树脂,进一步研究其纯化多酚的工艺条件和技术参数。结果显示:上样溶液质量浓度为25 mg/m L,上样流速为1 m L/min,以10 BV进行上样,以50%的乙醇溶液为解析剂,洗脱流速为3 m L/min,洗脱体积为8 BV进行洗脱,栘叶浸膏中多酚的含量由原来的39.7%提高到91.89%。纯化后,栘叶多酚对胰淀粉酶的抑制率高于纯化前,且纯化前后对胰淀粉酶的抑制率均高于100 mg/m L大黄提取物对胰淀粉酶的抑制效果,说明栘叶多酚对胰淀粉酶有明显的抑制效果,且纯化有利于提高栘叶多酚对胰淀粉酶的抑制作用。To propose an optimal purification process for polyphenol extracted from Docyniaindica （Wall.）Dcne leaves and investigate the inhibition of pancreatic amylase before and after purification.Macroporous resins D-101 was found more suitable to purify polyphenols by comparing their static adsorption and desorption capacities with those of two other kinds of macroporous resin.The purification process of D-101 was determined after static and dynamic experiment.When the D-101 was adsorbed with the maximum sample volume of 10 BV sample solution containing 25 mg/mL extractive at a rate of 1 mL/min, and desorpted with 8 BV of 50% ethanol at a flow rate of 3 mL/min,the content of the total polyphenol could be increased from 39.7% to 91.g9%.The method proved to be simple and feasible for purification of total polyphenols extracted from Docyniaindica（Wall.）Dcne leaves. After purification,the inhibitory rate of total polyphenols extracted from Docyniaindica（Wall.）Dcne leaves increased, and the inhibition rate before and after purification was higher than that of 100 mg/mL rhubarb extract on pancreatic amylase, indicating that polyphenols of Docyniaindica （Wall.）Dcne leaves had obvious inhibiting effect on pancreatic amylase,which the purification can improve.

关 键 词：栘叶 多酚 大孔吸附树脂 纯化 胰淀粉酶 

分 类 号：TS201.1[轻工技术与工程—食品科学]