复合重组磷脂酶用于大豆油脱胶的工艺优化  

Optimization of Soybean Oil Degumming by Mixed Recombinant Phospholipases

作　　者：程实[1,2] 王长坤[1,2] 张梁[1,2] 李赢[2] 石贵阳[2] 杨盛荣 陈国安 

机构地区：[1]江南大学工业生物技术教育部重点实验室,江苏无锡214122 [2]江南大学粮食发酵工艺与技术国家工程实验室,江苏无锡214122 [3]无锡江大百泰科技有限公司,江苏无锡214122

出　　处：《食品科学》2016年第18期13-18,共6页Food Science

基　　金：国家高技术研究发展计划(863计划)项目(2011AA100905);教育部"新世纪优秀人才支持计划"项目(NCET-11-0665);江南大学食品科学与技术国家重点实验室自由探索课题项目(SKLF-ZZA-201201)

摘　　要：利用实验室前期构建的重组大肠杆菌所产磷脂酶A_1(phospholipase A_1,PLA_1)和磷脂酶C (phospholipase C,PLC)进行大豆油酶法脱胶研究,探讨自主开发重组酶进行酶法脱胶的可行性。以诺维信商品酶Lecitase Ultra^(TM)为对照,研究酶法脱胶反应温度、反应pH值、反应时间、搅拌速率、复合磷脂酶添加量工艺参数对大豆油脱胶的影响,并采用正交试验对脱胶工艺条件进行优化。研究结果表明,大豆油复合酶法脱胶的最佳工艺参数为:反应温度45℃、反应pH 6.5、反应时间3 h、搅拌速率300 r/min、PLA_1和PLC添加量分别为7 940 U/kg和23 130 U/kg。复合磷脂酶对大豆油脱胶的效果与诺维信商品酶Lecitase Ultra^(TM)基本一致,大豆油磷含量可降至5 mg/kg以下,能够满足物理精炼的要求,为进一步开发具有知识产权的食品级油脂脱胶用酶制剂产品提供了理论依据。Crude soybean oil was degummed by mixtures of recombinant phospholipases A1（PLA1） and phospholipase C（PLC）, which were expressed successfully in the recombinant E. coli. by our laboratory. We investigated the effects of different parameters, including temperature, pH, reaction time, water dosage, stirring rate and enzyme dosage on degumming efficiency. Commercial PLA1（Lecitase Ultra^TM） was used for comparison. Then, these parameters were optimized by orthogonal array design. The optimal conditions were confirmed as follows： reaction temperature, 45 ℃; pH, 6.5; reaction time, 3 h; stirring speed, 300 r/min; PLA_1 dosage, 7 940 U/kg; and PLC dosage, 23 130 U/kg. The degumming efficiency obtained with the mixed recombinant enzymes was comparable to that obtained with Lecitase Ultra^TM. The final phosphorus content of degummed soybean oil was lower than 5 mg/kg, which met the requirements of physical refining. This study laid the foundation for developing food-grade enzyme preparations with self-owned intellectual property rights of cooking oil degumming.

关 键 词：重组大肠杆菌 复合磷脂酶 大豆油 酶法脱胶 正交试验 

分 类 号：Q814.9[生物学—生物工程]