高效液相色谱法同时测定豆浆中麦芽酚、乙基麦芽酚、香兰素、甲基香兰素和乙基香兰素5种香料  

Simultaneous determination of maltol,ethyl maltol,vanillin,methyl vanillin and ethyl vanillin in soya bean milk by high performance liquid chromatography

作　　者：迟秋池 李晓雯[1] 何家今 王柯[1] 刘畅[1] CHI Qiu-Chi LI Xiao-Wen HE Jia-Jin WANG Ke LIU Chang(Shanghai Institute for Food and Drug Control, Shanghai 201203, China)

机构地区：[1]上海市食品药品检验所,上海201203

出　　处：《食品安全质量检测学报》2016年第7期2690-2695,共6页Journal of Food Safety and Quality

摘　　要：目的建立高效液相色谱法同时测定豆浆中麦芽酚、乙基麦芽酚、香兰素、甲基香兰素和乙基香兰素5种香料的含量。方法样品经沉淀蛋白后用水稀释,采用Thermo Hypersil-Gold C（18）色谱柱分离,以0.1%甲酸溶液-甲醇为流动相进行梯度洗脱,在273 nm波长下进行高效液相色谱检测。结果在1-100μg/g浓度线性范围内,麦芽酚、乙基麦芽酚、香兰素、甲基香兰素和乙基香兰素呈现良好的线性关系,相关系数大于0.999,在2、5、10和20μg/g 4个添加水平下,加标回收率为63.39%-88.73%,RSD为0.9%-4.5%（n=6）,检出限在0.04-0.18μg/g之间。结论该方法灵敏度高、重复性好,可应用于实际样品的测定。Objective To establish a method for simultaneous determination of maltol, ethyl maltol, vanillin, methyl vanillin and ethyl vanillin in soya bean milk by high performance liquid chromatography （HPLC）. Methods After protein deposition, the samples were diluted by water, separated by Thermo Hypersil-Gold C18 column with the mobile phase of 0.1% formic acid-methanol for gradient elution, and detected at the wavelength of 273 nm by HPLC. Results Maltol, ethyl maltol, vanillin, methyl vanillin and ethyl vanillin had good linear relationships in the range of 1-100 μg/g with correlation coefficients higher than 0.999. The individual average recoveries spiked at 4 levels of 2, 5, 10 and 20 μg/g were ranged from 63.39% to 88.73%, while the relative standard deviations （RSD） were 0.9%-4.5% （n=6）. The limits of detection were 0.04-0.18 μg/g. Conclusion The established method has high sensitivity and good repeatability, which can be applied for testing actual samples.

关 键 词：麦芽酚 乙基麦芽酚 香兰素 甲基香兰素 乙基香兰素 豆浆 高效液相色谱法 

分 类 号：O657.72[理学—分析化学] TS214[理学—化学]