果胶-壳聚糖多层修饰脂质体的制备及配方优化  

Preparation of pectin-chitosan layer by layer modified liposomes and the formula optimization

作　　者：白春清[1] 郑景霞[2] 袁璐[1] 崔丹丹[1] 陈丽丽[1] 袁美兰[1] 赵利[1] BAI Chun-qing ZHENG Jing-xia YUAN Lu CUI Dan-dan CHEN Li-li YUAN Mei-lan ZHAO Li(College of Life Science ,Jiangxi Science and Technology Normal University, Nanchang 330013, China Nanchang University, State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China)

机构地区：[1]江西科技师范大学生命科学学院国家大宗淡水鱼加工技术研发分中心,江西南昌330013 [2]南昌大学食品科学与技术国家重点实验室,江西南昌330047

出　　处：《食品工业科技》2016年第21期49-53,59,共6页Science and Technology of Food Industry

基　　金：国家自然科学基金地区项目(31560465);江西省自然科学基金资助项目(20151BAB214023;20161BAB204190);江西省大宗淡水鱼产业技术体系项目(JXARS-04-赣中片);江西省教育厅青年项目(GJJ150803)

摘　　要：本研究利用壳聚糖、果胶对常规脂质体进行先后修饰构建果胶-壳聚糖多层修饰脂质体(pectin-chitosan coated liposomes,Pe-Ch-Liposomes),以番茄红素为模型药物,系统研究了主要因素对多层修饰脂质体包封率和浊度的影响,并以包封率为指标优化配方。结果表明果胶溶液与壳聚糖溶液的体积及浓度配比均对多层修饰脂质的包封率及浊度有一定的影响,且多层修饰过程可降低由卵磷脂与胆固醇质量比引起的包封率及浊度的差异。正交实验结合单因素分析确定最佳配方为果胶壳聚糖体积比为2∶1,果胶-壳聚糖总浓度为0.2%∶0.2%,卵磷脂与胆固醇质量比为5∶1,番茄红素添加量为2.5 mg。在此条件下制备的多层修饰脂质体的包封率(97.8%)显著高于常规脂质体的包封率(85.4%);粒度分布较为集中,平均粒径为1870.4 nm,多分散系数为0.231。In this research pectin-chitosan layer by layer modified liposomes were successfully prepared. The effects of main factors on the encapsulation efficiency and turbidity were evaluated, and optimal formula was obtained by orthogonal experiments using encapsulation efficiency as the main index.The results indicated that the volume ratio and concentration ratio of pectin solution to chitosan solution had significant effects on the encapsulation efficiency and turbidity of pectin-chitosan layer by layer modified liposomes. The process of modification could diminish the properties differences affected by the mass ratio of lecithin to cholesterol. The optimal formula was the volume ratio of pectin solution to chitosan solution 2： 1, concentrations of pectin solution and chitosan solution 0.2%：0.2% ,the mass ratio of lecithin to cholesterol 5： 1, addition amount of lycopene 25 mg. The encapsulation efficiency of the optimized pectin-chitosan modified liposomes was significantly higher than that of conventional liposomes, which was 97.8% and 85.4% for them, respectively,the particle size distribution of the obtained pectin- chitosan modified liposomes was narrow with the mean particle size of 1870.4 nm and poiydispersity index of 0.231.

关 键 词：多层修饰脂质体 番茄红素 果胶 壳聚糖 

分 类 号：TS218[轻工技术与工程—粮食、油脂及植物蛋白工程]