葫芦巴超临界CO_2提取物的GC-MS分析及对DPPH自由基的清除作用  

Analysis of chemical components by GC-MS and scavenging capacity against DPPH free radical of SFE extracts from Trigonella foenum-graecum L.

作　　者：项昭保[1,2] 

机构地区：[1]重庆工商大学环境与资源学院,重庆400067 [2]天然药物研究重庆高校市级重点实验室,重庆400067

出　　处：《食品工业科技》2016年第24期153-156,162,共5页Science and Technology of Food Industry

基　　金：天然药物研究重庆市高校市级重点实验室开放基金项目(CQCM-2015-07);重庆工商大学科研启动项目(2015-56-07);重庆工商大学科研基金项目(2015)

摘　　要：采用超临界二氧化碳提取葫芦巴提取物,利用气相色谱-质谱法分析提取物的化学成分,并通过面积归一化法分析各成分的相对含量。从葫芦巴超临界二氧化碳提取物中共鉴定出49个化合物,占提取物总量的88.37%,其中亚油酸(45.29%)含量最高。利用分光光度法测定葫芦巴超临界二氧化碳提取物对DPPH自由基的清除作用,其清除作用良好,半清除浓度为4.57 mg·m L-1,且清除率与样品浓度呈量效关系。葫芦巴超临界提取物营养成分丰富、安全无毒、有特殊香气,在食品、香料等领域可广泛应用。Supercritical fluid extraction（SFE）using carbon dioxide was employed to extract Trigonella foenum- graecum L.and the chemical components were determined by GC-MS. The relative contents of the chemical components were determined with peak area normalization method. Forty-nine compounds, which occupied 88.37% of total constituents were identified. The major constituent was linoleic acid （45.29%）. The scavenging capacity against DPPH free radical of SFE extracts from Trigonella foenum-graecum L.was determined by spectre photometry.The extracts exhibited significant scavenging capacity against DPPH free radical in a concentration- dependent fashion with the SC5o value of 4.57 mg·mL-1.The SFE extracts was rich in nutrients, safe and non-toxic, and had special aroma.So it could be widely used in the fields of food and perfume.

关 键 词：葫芦巴 超临界CO2提取 气相色谱-质谱联用 DPPH自由基 

分 类 号：TS201.2[轻工技术与工程—食品科学]