降胆固醇植物乳杆菌I4菌冻干保护剂的响应面优化研究  

Optimization of cryoprotectant of I4 cholesterol-lowering plant Lactobacillus bacteria using response surface method

作　　者：朱奇奇 张驰翔 王周[1] 焦士蓉[1] ZHU Qi-qi ZHANG Chi-xiang WANG Zhou JIAO Shi-rong(School of Bioengineering, Xihua University, Chengdu 610039)

机构地区：[1]西华大学生物工程学院,成都610039

出　　处：《食品科技》2016年第11期2-7,共6页Food Science and Technology

基　　金：西华大学省级重点试验室开放研究基金项目(szjj2014-009);西华大学研究生创新基金项目(ycjj2015025);教育部春晖计划项目(12205543)

摘　　要：为了提高直投式降胆固醇植物乳杆菌I4菌发酵剂的生物活性,考察了5种保护剂对I4菌真空冷冻干燥的保护效果,选择其中效果较好的4种保护剂进行响应面优化,结果表明,以4.04%海藻糖、2.9%谷氨酸钠、10.38%蔗糖、14.84%脱脂乳粉作为复合保护剂,I4的冻干存活率较高,可达83.32%。通过对冻干后I4菌发酵剂降胆固醇能力的测试,得出胆固醇降解率为(36.2±1.75)%(n=3)。In order to improve the direct investment type of I4 cholesterol-lowering plant Lactobacillus bacteria starter cultures of biological activity. The five kinds of protective agent of vacuum freeze-drying protective effect of I4 bacteria were studied. Four protective agent with better effect were optimized by response surface method. The result showed that the optimal freeze-drying protective material contained 4.04% trehalose, 2.9% sodium glutamate, 10.38% sucrose, 14.84% dried skim milk, the survival rate reached 83.32%. The test of the cholesterol-lowering ability of the formula indicated a degrading rate of（36.2±1.75）%（n=3）.

关 键 词：植物乳杆菌 真空冷冻干燥 响应面优化 冻干保护剂 

分 类 号：TS201.3[轻工技术与工程—食品科学]