油橄榄果渣水不溶性膳食纤维结构表征及体外吸附性能研究  

Structural characterization and in-vitro adsorption capacity of olive pomace insoluble dietary fiber

作　　者：丁莎莎[1] 黄立新[1] 张彩虹[1,2] 谢普军[1] 张琼[1] 邓叶俊 DING Sha-sha HUANG Li-xin ZHANG Cai-hong XIE Pu-jun ZHANG Qiong DENG Ye-jun(Institute of Chemical Industry of Forest Products,CAF,National Engineering Lab.for Biomass Chemical Utilization, Key and Open lab.of Forest Chemical Engineering,SFA,Key Lab.of Biomass Energy and Material, Jiangsu Province, Nanjing 210042, China Research Institute of Forestry New Technology, CAF, Beijing 100091, China)

机构地区：[1]中国林业科学研究院林产化学工业研究所生物质化学利用国家工程实验室国家林业局林产化学工程重点开放性实验室江苏省生物质能源与材料重点实验室,江苏南京210042 [2]中国林业科学研究院林业新技术研究所,北京100091

出　　处：《食品工业科技》2017年第3期108-112,共5页Science and Technology of Food Industry

基　　金：中国林业科学研究院中央级公益性科研院所基本科研业务费专项资金(CAFYBB2016QA011)

摘　　要：以油橄榄脱脂果渣为原料,采用碱法制备油橄榄果渣水不溶性膳食纤维(Insoluble dietary fiber,IDF),并用红外光谱和X射线衍射等方法对其表征,并测定分析其体外吸附NO-2和重金属Pb^(2+)、Cu^(2+)、Cd^(2+)的功能特性。结果表明:油橄榄果渣IDF中水不溶性膳食纤维含量为90.09%,其主要由纤维素、半纤维素和木质素组成;红外光谱图显示油橄榄果渣IDF具有糖类和木质素的特征吸收峰;X射线衍射图显示油橄榄果渣IDF呈纤维素I晶型,其结晶度为47.94%;在体外模拟胃环境(pH2)下,油橄榄果渣IDF对NO_2^-的吸附在240 min时达到平衡,平衡吸附量为826μg/g;在体外模拟肠道环境(pH7)下,油橄榄果渣IDF对Pb^(2+)、Cu^(2+)、Cd^(2+)的平衡吸附量分别为418.7、686.6、849.5μg/g,IDF在肠道环境下对Pb^(2+)、Cu^(2+)、Cd^(2+)的吸附能力均优于胃环境。IDF对Pb^(2+)、Cu^(2+)、Cd^(2+)的吸附过程符合准二级动力学方程。Olive pomaee insoluble dietary fiber（IDF） was prepared by alkaline method with defatted olive pomaee.In addition to its chemical composition and structure characterized by IR and X-ray diffraction ,the effect of olive pomace IDF on the in vitro adsorption capacity of NO2^-, Pb^2＋ , Cu^2＋ , Cd^2＋ was studied.These results showed that the IDF content in olive pomace IDF reached 90.09% ,which was mainly comprised by cellulose, hemicellulose and lignin.The result of IR showed that olive pomace IDF appeared characteristics absorption peaks of sugars and lignin. The result of X-ray diffraction indicated that IDF had cellulose I crystal structure, and its crystallinity achieved 47.94% .The quantities of equilibrated adsorption to NO2^- using IDF under the simulated gastric condition（pH2） was attained 826 μg/g at 240 min.The adsorption capacity of IDF tot Pb^2＋ , Cu^2＋ , Cd^2＋ under the simulated intestine condition （pH7）was far stronger than that in the gastric. The equilibrated adsorption quantities for Pb^2 ＋ , Cu^2＋ , Cd^2 ＋ in simulated intestine condition could reach 418.7,686.6,849.5μg/g, respectively.Besides, the pseudo-second-order kinetics equations were exactly fit for those adsorption processes of IDF for Pb^2＋ , Cu^2＋ ,Cd^2＋.

关 键 词：油橄榄果渣 水不溶性膳食纤维 表征 NO2^- 重金属 

分 类 号：TS201.1[轻工技术与工程—食品科学]