高效液相色谱法测定火锅汤底中的咖啡因  

Determination of caffeine in hot pot soup by high performance liquid chromatography

作　　者：李聪[1] 邱启东[1] 李锦清[1] 张施敬[1] 陈海燕[1] 李晓明[1] 

机构地区：[1]广东产品质量监督检验研究院,国家食品质量监督检验中心(广东),佛山528300

出　　处：《食品安全质量检测学报》2016年第12期4772-4775,共4页Journal of Food Safety and Quality

基　　金：广东省科技计划科研项目(2016A040403071)~~

摘　　要：目的建立高效液相色谱法(high performance liquid chromatography,HPLC)测定火锅汤底中咖啡因的含量。方法样品在碱性条件下经三氯甲烷提取,采用Phenomenex C_(18)色谱柱分离,以甲醇:水:乙酸=10:89:1(V:V:V)为流动相等度洗脱,流速为1.0 m L/min,柱温为40℃。采用二极管阵列检测器在286 nm波长下进行检测,以外标法定量。结果咖啡因在1~100 mg/kg浓度范围内具有良好的线性关系,相关系数为0.9990,检出限为2.00×10^(-3) mg/kg,在20.00和50.00 mg/kg 2个添加水平上的加标回收率为87.0%~93.6%,相对标准偏差为3.01%~5.91%。结论该方法操作简单,灵敏度高,重复性好,可适用于火锅汤底中咖啡因的测定。Objective To establish a method for determination of caffeine in hot pot soup by high performance liquid chromatography （HPLC）.Methods Samples were extracted with trichloromethane under the alkaline condition, separated by Phenomenex C18 column with methyl alcohol：water：acetic acid （10：89：1,V：V：V） as mobile phase for isocratic elution at a flow rate of 1.0 mL/min at 40℃, and then detected by diode array detector at 286 nm. The quantification was performed by the external standard method.Results Caffeine had a good linear relationship in the range of 1~100 mg/kg with correlation coefficients（r2） of 0.9990. The detection limit was 2.00×10-3mg/kg. The average recoveries for caffeine spiked at 2 levels of 20.00 and 50.00 mg/kg were in the range of 87.0%~93.6% with relative standard deviations （RSDs） of 3.01%~5.91%.Conclusion With the advantages of simplicity, sensitivity and repeatability, the established method can meet the requirements for determination of caffeine in hot pot soup.

关 键 词：高效液相色谱法 火锅汤底 咖啡因 

分 类 号：TS264.2[轻工技术与工程—发酵工程] O657.72[轻工技术与工程—食品科学与工程]