竹荪中多酚提取工艺优化及其抗氧化活性研究  

Study on the extraction and antioxidant activity of total phenolic compounds from Dictyophora indusiata

作　　者：吴林秀[1,2] 胡荣康[1,2] 陈艺煊 王娟娟[1] 刘晓艳[1,2] 刘斌[1,2] 

机构地区：[1]福建农林大学食品科学学院,福建福州350002 [2]国家菌草工程技术研究中心,福建福州350002

出　　处：《食品工业科技》2017年第7期203-206,215,共5页Science and Technology of Food Industry

基　　金：国家科技支撑计划子课题(2014BAD15B01-6);福建省科技重大专硕(2014NZ2002-1);福建农林大学青年基金课题(k13xjj17a)

摘　　要：本文以长裙竹荪为原料,以多酚得率为考察指标,通过乙醇浓度、料液比、提取温度和提取时间4个单因素实验和正交实验确定竹荪多酚的最佳提取条件;并且通过竹荪提取液对2,2-二苯代苦味酰基(DPPH)自由基和羟基自由基的清除效果,评价竹荪提取液的体外抗氧化活性。结果表明:在乙醇浓度为40%、提取温度为80℃、料液比为1∶40 g/m L、提取时间为5 h的条件下,竹荪多酚的得率最高,可达(8.18±0.52)mg/g;竹荪提取液呈现出较好的抗氧化活性,且与浓度存在一定的量效关系,在质量浓度为20 mg/m L时,提取液对DPPH自由基的清除率可达84.28%±1.15%,对羟基自由基清除率达到76.49%±1.14%。The contents of polyphenols from Dictyophora indusiata were determined by the Folin-Ciocaheu method in the paper.The effects of optimal alcohol concentration, liquid-solid ratio, extraction time and extraction temperature yield of D.indusiata were investigated, and the extraction technology was optimized by single-factor experiments and orthogonal design. The antioxidant activity of D.indusiata phenols was also studied.The results showed that the optimal extraction conditions were found that the optimal alcohol concentration, solidliquid ratio, extraction time and extraction temperature were 40%, 1 ： 40 g/mL, 80 ℃ and 5 h respectively.Under these conditions, the largest extraction quantity of total phenols could reach （ 8.18 ±0.52） mg/g.The DPPH radical scavenging ratio and · OH radicals of the polyphenol extracts reached 84.28% ± 1.15% and 76.49%±1.14% respectively when the extract concentration was 20 mg/mL.

关 键 词：竹荪 多酚 抗氧化性 正交设计 

分 类 号：TS201.1[轻工技术与工程—食品科学]