产朊假丝酵母核酸高产株的选育及其发酵性能研究  

Breeding of a Candida utilis Mutant for Efficient Nucleic Acid Production and its Fermentation Performance

作　　者：蒋雪薇[1] 岳希洁 陈代文 周志明 罗晓明[1] 叶菁[1] 李晓文[1] 

机构地区：[1]长沙理工大学化学与生物工程学院,湖南长沙410004 [2]连南瑶族自治县奇乡生物科技有限公司,广东清远513300

出　　处：《食品科学》2017年第10期149-154,共6页Food Science

基　　金：清远市科技计划项目(2013A024;2014A023);长沙市科技计划重大专项(kq1601013)

摘　　要：为了获得一株发酵性能优良的高核酸酵母,以产朊假丝酵母(Candida utilis)CL1501为出发菌株,采用紫外-亚硝基胍复合诱变,筛选获得一株高核酸含量的突变株CL15013,经测定其核酸含量占菌体干质量的16.8%,高于出发菌47.8%。对比研究其流加及分批培养工艺,流加培养细胞收获量为16.9 g/L,比分批培养提高94.3%;正交试验设计优化流加培养条件后,CL15013的收获量达21.3 g/L,比分批培养提高144.8%,具有良好的生产应用潜力。CL 1501 was mutagenized with a combination o f UV irradiation and nitrosoguanidine treatment.An excellent mutant with high ability to produce nucleic acid, named as CL15013, was obtained. Its nucleic acid content was 16.8% on a dry cell weight basis,which was 47.8% higher than that of CL1501. The dry biomass yield of the mutant was 16.9 g/L in fed-batch culture, which was 94.3% higher than that from batch culture. Under optimized fed-batch culture conditions obtained by orthogonal array design, the biomass yield was up to 21.3 g/L, which was 144.8% higher than that from batch cultivation. These results indicated that mutant CL 15013 has a great application potential in industrial production.

关 键 词：产朊假丝酵母 复合诱变 核酸 流加培养 

分 类 号：TQ926.1[轻工技术与工程—发酵工程]