多指标评价核桃蛋白及多肽的抗氧化活性  

Studies on the Antioxidant Activity of Walnut Protein and Polypeptide by Multi-index

作　　者：李丽[1,2] 阮金兰[1,2] 钱伟亮 周涵黎 王亚云[1,2] 吴娜[1,2] 刘阳[1,2] 

机构地区：[1]武昌理工学院生命科学学院,生物多肽糖尿病药物湖北省协同创新中心,湖北省生物多肽糖尿病药物工程技术研究中心,湖北武汉430223 [2]武汉工程大学化工与制药学院,湖北武汉430073

出　　处：《食品研究与开发》2017年第3期1-4,共4页Food Research and Development

基　　金：湖北省自然科学基金资助项目(2013CFB482);湖北省大学生创新训练项目(201312310012);武昌理工学院校级项目(2015X04)

摘　　要：多指标评价药食两用核桃蛋白及多肽的抗氧化活性。利用DPPH·﹑O_2^-·﹑总还原能力和·OH 4种常用体外抗氧化活性模型,以谷胱甘肽(GSH)为对照,测定核桃蛋白及多肽抗氧化活性。在实验范围内,核桃蛋白及多肽的抗氧化活性与浓度呈量效关系,且核桃多肽对DPPH·和O_2^-·清除能力显著,抗氧化活性达到GSH的80%以上,对·OH清除能力亦达到GSH的50%以上。核桃蛋白及多肽均具有一定的抗氧化能力,且核桃多肽的抗氧化活性较蛋白更为显著。To evaluate the antioxidant activity of walnut protein and polypeptide. Compared with GSH,the antioxidant activity of walnut protein and polypeptide was measured by using the DPPH radical scavenging capacity,superoxide anion radical scavenging capacity,deoxidization capacity,and hydroxyl radical scavenging capacity. Within the experimental concentration,the antioxidant activity of walnut protein and polypeptide showed a dose-dependent manner. Moreover,the walnut polypeptide significantly exhibited DPPH radical scavenging capacity and superoxide anion radical scavenging capacity,with more than 80 % of GSH,and the hydroxyl radical scavenging ability of polypeptide reached more than 50 % of GSH. Walnut protein and polypeptide were deter mined to have obvious antioxidant activity,and the antioxidant activity of walnut polypeptide was more significant.

关 键 词：核桃蛋白 核桃多肽 抗氧化活性 谷胱甘肽 

分 类 号：TS201.2[轻工技术与工程—食品科学]