高酸苹果多酚鉴定及抗氧化、抗溶血活性研究  

Investigation into Identification,Antioxidant Activity and Anti-Hemolysis Activity of Phenolics Compounds in High Acid Apple

作　　者：孙为正[1] 杨坤[1] 林恋竹[1] 邹颖[1] 赵谋明[1] SUN Wei-zheng YANG Kun LIN Lian-zhu ZOU Ying ZHAO Mou-ming(School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, Guangdong, China)

机构地区：[1]华南理工大学食品科学与工程学院,广东广州510640

出　　处：《华南理工大学学报（自然科学版）》2017年第3期146-152,共7页Journal of South China University of Technology(Natural Science Edition)

基　　金：广东省自然科学基金博士启动基金资助项目(2015A030310494);广东"特支计划"科技创新青年拔尖人才项目(2014TQ01N538)~~

摘　　要：对新疆产高酸苹果—"洪勋1号(红肉)"进行系统研究,发现其可滴定酸度为1.31%,总酚和花青素含量依次为果皮>果渣>全果(总酚含量为(2026.88±28.30)mg/kg;花青素含量为(244.92±13.88)mg/kg)>果肉.采用高效液相色谱法对高酸苹果多酚进行分离鉴定,共鉴定出10种多酚类化合物,包括绿原酸、根皮苷、表儿茶素、阿魏酸、杨梅酮、儿茶素、香草酸、p-香豆酸、芦丁和咖啡酸,全果中以绿原酸(391.19±18.37)mg/kg)、表儿茶素(698.82±17.21)mg/kg)和儿茶素(570.30±9.21)mg/kg)为主.采用DPPH自由基清除法、氧自由基吸收能力法、2,2-偶氮二异丁基脒二盐酸盐(AAPH)损伤人血红细胞氧化模型评价苹果各部位的抗氧化活性与抗溶血活性,结果表明:高酸苹果的皮、肉、渣、全果均具有较强的抗氧化活性,抗溶血能力依次为果皮>果渣>全果>果肉.研究表明,高酸苹果值得深度开发加工为高营养价值的产品.The composition of a kind of high acid apple from Xinjiang, China, namely Hongxun I （red flesh ） , was determined, finding that the high acid apple is of a titratable acidity of 1. 31% , a total phenolics and anthocya- nins content in an order of peel 〉 pomace 〉 whole fruit （with a total phenolics content of （2026. 88 ±28. 30） mg/kg and a total anthocyanins content of （244. 92 ± 13. 88） mg/kg） 〉 flesh. Then, ten phenolic compounds including chlorogenic acid, phlorizin, epicatechin, ferulic acid, myricetin, catechins, vanillic acid, p-coumaric acid, rutin and caffeic acid were indentified by means of high-performance liquid chromatography, finding that the high acid apple is rich in chlorogenic acid （ （391. 19 ± 18. 37） mg/kg） , epicatechin （ （698. 82 ± 17. 21 ） mg/kg） and catechins （ （570. 30 ± 9. 21） mg/kg） . Moreover, the antioxidant and anti-hemolysis activities of the high acid ap-ple were determined by means of DPPH radical scavenging activity assay, oxygen radical absorption capacity assay and AAPH-induced oxidative damage human erythrocytes model, finding that the peel, flesh, pomace and whole fruit of the high acid apple all exhibit excellent antioxidant activity and that the anti-hemolysis activities are indica-tive of the following order peel 〉 pomace 〉 whole fruit 〉 flesh. All these above-mentioned statements indicate that, as a valuable product, high acid apple is worth processing and developing.

关 键 词：高酸苹果 抗氧化活性 抗溶血活性 多酚 

分 类 号：TS201[轻工技术与工程—食品科学]