发酵条件对胆固醇降解能力的影响  

Effects of fermentation conditions on cholesterol degradability

作　　者：戴颖[1] 瞿恒贤 瓦云超 黄玉军[1] 陈霞[1] 陈大卫[1] 杨正泉[1] 顾瑞霞[1] 

机构地区：[1]扬州大学食品科学与工程学院,江苏省乳品生物技术与安全控制重点实验室,江苏扬州225001

出　　处：《食品工业科技》2017年第12期123-128,共6页Science and Technology of Food Industry

基　　金：国家自然科学基金(31571855);“十二五”国家科技支撑计划项目(2013BAD18B12);江苏省科技支撑项目(BN2014080);江苏省高校自然科学研究重大项目(12KJA550003)

摘　　要：通过对混合菌株发酵条件进行优化,提高混合菌株胆固醇降解能力与活菌数,旨在获得高效降胆固醇的混合菌株,为乳酸菌应用提供依据。选取四株具有辅助降胆固醇活性乳酸菌将其1∶1∶1∶1混合,并通过单因素实验和响应曲面分析方法,以胆固醇降解率和活菌数为指标对混合菌株培养基和培养条件进行了优化。以MRS培养基为基础,更换不同碳氮源,确定混合菌株最适发酵条件为:乳糖24.19 g/L、胰蛋白胨8.41 g/L,培养温度28.28℃,培养时间30 h,接种量5.24%。经优化后,混合菌株胆固醇降解率由39.56%提高到53.43%,增加了35.06%;活菌数由8.87 lg CFU/m L提高到9.63 lg CFU/m L,与优化前相比上升了一个数量级。In order to improve the ability of cholesterol degradation and the number of viable bacteria, the study optimized the fermentation conditions of mixed strains, aiming at obtaining high efficient cholesterol-degrading strains and providing the basis for the application of lactic acid bacteria. Four strains of lactic acid bacteria with auxiliary cholesterol- lowering activity were selected and mixed with 1： 1： 1：1 mixture.The single factor experiment and response surface analysis were used to study the effects of cholesterol degradation rate and viable count.Conditions were optimized.The optimum fermentation conditions were as follows ： lactose 24.19 g/L, tryptone 8.41 g/L, culture temperature 28.28 ℃ , culture time 30 h, inoculation amount 5.24%.After optimization, the degradation rate of cholesterol in the mixed strains increased from 39.56% to 53.43 % , increased by 35.06%. The totol colonies was increased from 8.87 lgCFU/mL to 9.63 lgCFU/mL.The viable count was an order of magnitude higher than that before optimization.

关 键 词：混合乳酸菌 降胆固醇 发酵条件 响应面分析法 

分 类 号：TS201.3[轻工技术与工程—食品科学]