来源于腌干鱼的乳酸菌中抗氧化酶及胞外多糖研究  

Study on antioxidant enzymes and exopolysaccharides of lactic acid bacteria separated from salt-dried fish products

作　　者：蔡秋杏[1,2,3] 吴燕燕[1] 李来好[1] 杨贤庆[1] 赵永强[1] 王悦齐[1,3] 

机构地区：[1]中国水产科学研究院南海水产研究所,农业部水产品加工重点实验室,广东省渔业生态环境重点开放实验室,广东广州510300 [2]钦州学院食品工程学院,广西高校北部湾特色海产品资源开发与高值化利用重点实验室,广西钦州535099 [3]中国海洋大学食品科学与工程学院,山东青岛266000

出　　处：《水产学报》2017年第6期952-961,共10页Journal of Fisheries of China

基　　金：国家现代农业产业技术体系专项(CARS-50);国家自然科学基金(31571869);广东省海洋渔业科技与产业发展专项(A201501C02)~~

摘　　要：为研究从腌干鱼中获取的乳酸菌在细胞内外起抗氧化作用的主要活性物质,通过测定菌株胞内的超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)和过氧化氢酶(CAT)活性以及提取胞外多糖(EPS)并测定其抗氧化性,利用超高效液相色谱法(UPLC)分析其结构。结果显示,9株菌的SOD、GSH-Px和CAT的最高活性分别为44.67、15.26和1.23 U/mg prot,对应菌株为L21、L4和L21;9株菌的EPS产量为(5.71±0.18)^(50.33±1.89)mg/L,L21的EPS最高纯度为56.16%±1.08%。L21中EPS的DPPH自由基清除能力和还原能力较高,综合抗氧化能力相对较强,结构分析发现其含有甘露糖(Man)、鼠李糖(Rha)、葡萄糖(Glu)和半乳糖(Gal)等4种单糖。研究表明,分离自腌干鱼的乳酸菌中,抗氧化能力越强的菌株细胞内表现出越高的抗氧化酶活性,同时细胞外EPS的产量和纯度也较高,并且EPS也具有较强的抗氧化能力。从中筛选出一株综合抗氧化能力最强的L21,可以进一步作为生物源性抗氧化剂。In order to study the main active material with anti-oxidative effects of lactic acid bacteria（LAB） from salt-dried fish, the intracellular antioxidant enzymes and extracellular exopolysaccharides（EPS） were investigated.Vitality of superoxide dismutase（T-SOD）, glutathione peroxidase（GSH-Px） and catalase（CAT） of nine strains were determined and the highest activity were 44.67, 15.26, and 1.23 U/mg prot respectively, which was corresponding to L21, L4 and L21. The crude EPS of LAB was prepared, and the production of nine LABs were between（5.71±0.18）–（50.33±1.89） mg/L, the highest purity of（56.16%±1.08%） was showed in a Lactobacillus casei（L21）. The antioxidant capacity of EPS was then investigated by 1,1-diphenyl-2-picryhydrazyl（DPPH）radical scavenging and reducing power. The compositive antioxidant capacity was the highest in L21. Finally the structure of its EPS was analysed using ultra performance liquid chromatography（UPLC）. The EPS of L21 contained four kinds of monosaccharides, namely, mannose（Man）, rhamnose（Rha）, glucose（Glu） and galactose（Gal）. Thus, the nine LABs with high antioxidative capacity isolated from salt-dried fish showed higher antioxidant enzyme activity, and the production and purity of EPS were also high, and could show a certain antioxidant capacity in EPS. Therefore, the strongest comprehensive antioxidant strain was L21, and it could be further used as a biological antioxidant.

关 键 词：乳酸菌 腌干鱼 超氧化物歧化酶 谷胱甘肽过氧化物酶 过氧化氢酶 胞外多糖 

分 类 号：TS201.3[轻工技术与工程—食品科学]