固相萃取柱净化-液相色谱-串联质谱法测定糕点中脱氧雪腐镰刀菌烯醇及其衍生物和玉米赤霉烯酮  

Determination of deoxynivalenols and zearalenone in pastry by solid phase extraction coupled with liquid chromatography-tandem mass spectrometry

作　　者：王丽娟[1,2] 柯润辉[1,2] 安红梅[1,2] 尹建军[1,2] 宋全厚[1,2] 

机构地区：[1]中国食品发酵工业研究院,北京100015 [2]国家食品质量监督检验中心,北京100015

出　　处：《食品工业科技》2017年第14期31-34,50,共5页Science and Technology of Food Industry

摘　　要：建立使用超高效液相色谱-串联质谱仪测定糕点中玉米赤霉烯酮(ZEN)、脱氧雪腐镰刀菌烯醇(DON)及其衍生物3-乙酰基-脱氧雪腐镰刀菌烯醇(3-Ac DON)、15-乙酰基-脱氧雪腐镰刀菌烯醇(15-Ac DON)4种真菌毒素的方法。该方法在4 min内完成4种真菌毒素的分析,线性范围5~1000μg/kg,标准曲线的相关系数均在0.997以上,DON、ZEN的最低检出限为1.0μg/kg,3-Ac DON、15-Ac DON的最低检出限为1.5μg/kg。通过对134份市售预包装糕点样品进行分析发现,4种真菌毒素均有一定检出,其中ZEN、DON、3-Ac DON和15-Ac DON的检出率分别为2.2%、78.4%、3.0%和5.2%,所有样品中4种真菌毒素含量水平均未超过食品安全国家标准限量要求。A method for simultaneous determination of deoxynivalenol,3-Acetyl deoxynivalenol,15-Acetyl deoxynivalenol and zearalenone in pastry had been developed by high performance liquid chromatography-tandem mass spectrometry（ HPLC-MS/MS）.The determination was finished within 4 minutes.The results showed that all of the 4 mycotoxins had good linearity in the range of 5-1000 μg/kg with the correlation coefficients（ R^2） better than 0.997.The limits of detection（ LODs） of DON and ZEN were 1.0 μg/kg,3-Ac DON and 15-Ac DON were 1.5 μg/kg. The results of 134 pastry samples determined by the method showed that all of the 4 mycotoxins were detected in the tested samples.The detection rate were 2.2%,78.4%,3.0% and 5.2%for ZEN,DON,3-Ac DON and 15-Ac DON. The maximum residual contents were lower than national food safety limited requiements.

关 键 词：固相萃取 液相色谱-串联质谱仪 真菌毒素 糕点 

分 类 号：TS207.3[轻工技术与工程—食品科学]