小麦淀粉加工废水中阿拉伯木聚糖的理化性质及抗氧化活性研究  

Study on physicochemical properties and antioxidant activity of Arabinoxylan from wheat starch wastewater

作　　者：田贝贝[1] 陈洁[1] 王远辉[1] 

机构地区：[1]河南工业大学粮油食品学院,河南郑州450001

出　　处：《食品工业科技》2017年第15期40-44,共5页Science and Technology of Food Industry

基　　金：以小麦副产物生产阿拉伯木聚糖的优化合作研究项目(2015DFA30380)

摘　　要：从小麦淀粉加工废水中制备阿拉伯木聚糖(AX),通过激光光散射法和离子色谱法(IC)对其相对分子质量和单糖组分等理化性质进行分析,并采用体外实验研究AX的抗氧化性。研究表明:源自小麦淀粉废水的AX具有较强的还原能力和DPPH自由基清除能力,对羟基自由基清除作用则相对较弱。分级醇沉可以有效分离具有不同分支结构、单糖比例、相对分子质量的AX,其中60%乙醇醇沉AXE纯度较高,纯度为86.81%。分级醇沉AX,随乙醇浓度的增加,目标AX的平均相对分子质量减小,其Ara/Xyl比值及半乳糖(Gal)含量增加。具有不同相对分子质量、分支结构及单糖比例的AX,其对DPPH自由基、羟基自由基清除作用以及还原力不同,分子质量较大且具有较小分支结构的AX具有较强的供氢能力,而络合游离金属离子的能力相对较弱。Arabinoxylan were prepared from wheat starch wastewater.The physicochemical properties and antioxidant activity of AX were analyzed by laser light scattering method, IC and in vitro experiments. The results showed that the AX from wheat starch wastewater had a strong reducing ability and a scavenging capacity of DPPH free radicals, while the ability of scavenging hydroxyl radical of AX was relatively weak. Ethyl alcohol fractionation precipitation can effectively separate the AX with different degree of branching, monosaccharide ratio and molecular weight.The AXE （60% ethyl alcohol precipitates）had higher purity（86.81% ）.The galactose （Gal）content and ratio of Ara/Xyl of AX increased with the increasing concentrations of ethanol,while the molecular weight decreased.The molecular weight, branched structure and monosaccharide ratio of AX played an important role in antioxidant activity.The AX with low molecular weight and a higher degree of branching showed a greater capability to provide hydrogen atoms, while the ability to complex free metal ions was relatively weak.

关 键 词：小麦淀粉废水 阿拉伯木聚糖 相对分子质量 抗氧化性 

分 类 号：TS249.9[轻工技术与工程—制糖工程]