玉米降血糖活性肽制备分离及其氨基酸序列分析  

Preparation,separation and amino acid sequence analysis of corn peptides with hypoglycemic activity

作　　者：胡宇航[1,2] 戴军[1] 陈尚卫[1] 朱松[1] 张学军 张平 

机构地区：[1]江南大学食品科学与技术国家重点实验室,江苏无锡214122 [2]江南大学食品学院,江苏无锡214122 [3]山东天久生物技术有限公司,山东菏泽274108

出　　处：《食品与机械》2017年第6期147-152,共6页Food and Machinery

摘　　要：以水解度(DH)和游离氨基酸含量为指标优化玉米蛋白粉的酶水解工艺,结果显示,在pH 8.5,60℃条件下经过正交试验得到最适组合为:酶底物比3.5g/100g,水解时间2h,料液比120(g/mL),在该条件下其DH为27.02%,多肽含量为85.23%。同时,还优化了玉米肽的脱色工艺,最佳条件为:温度50℃,pH 3.5,活性炭用量1.5g/100mL,脱色时间45min,该条件下玉米肽得率为77.86%,脱色率为87.27%。将脱色脱盐后的玉米多肽经过凝胶色谱分离得到3个组分。体外降血糖活性结果显示,CP1促进正常HepG2细胞葡萄糖消耗效果最优,并且其α-糖苷酶抑制活性也最高(34.64%);CP1经过RP—HPLC制备柱纯化得到含量较高的14个组分,其中CP1-13的α-糖苷酶抑制活性最強,达到39.50%。经UPLC—Q—TOF—MS/MS测定,其氨基酸序列为A-P-A-L-L-P-F。In this study,the degree of hydrolysis（DH）and polypeptide content were index optimization for corn gluten meal of hydrolysis process.Under the condition of pH 8.5,60℃,orthogonal experiment to obtain the optimal combination for：amount of enzymeenzyme 3.5g/100 g,hydrolysis time 2h,solid-liquid ratio 1︰20（g/mL）;on this condition,the DHis 27.02%,peptide content is85.23%.Meanwhile,optimized the decolorization technology,the best conditions：temperature 50 ℃,pH 3.5,activated carbon dosage：1.5g/100 mL,decoloring time 45min;under the condition,corn peptide yield was 77.86%,the decolorization rate was 87.27%.After decoloring and desalination,three components were isolated by chromatographic separation.The result of hypoglycemic activity in vitro showed that CP1 had the better effect on promoting normal HepG2cells＇ glucose consumption,and theα-glycosidic enzyme inhibitory activity reached 34.64%,after RP-HPLC column purification,14 components were gotten;the thirteenth（CP1-13）had the bestα-glycosidic enzyme inhibitory activity,and reached 39.50%.Determined by UPLC-Q-TOF-MS/MS,the amino acid sequence was A-P-A-L-L-P-F.

关 键 词：玉米肽 水解 降血糖活性 氨基酸序列 

分 类 号：TS218[轻工技术与工程—粮食、油脂及植物蛋白工程]