低值小杂鱼酶解产物清除DPPH自由基能力的研究  

The DPPH Free Radical Scavenging Activities of Hydrolysate from Low-value Little Fish

作　　者：周燕芳 施秀杰 

机构地区：[1]韩山师范学院化学与环境工程学院,潮州521041

出　　处：《食品工业》2017年第9期39-42,共4页The Food Industry

基　　金：2015国家级星火计划项目(2015GA780048);韩山师范学院一般项目(LY201502)

摘　　要：以中性蛋白酶为水解酶,以酶解产物对DPPH自由基清除率为指标,利用正交试验优化制备具有体外抗氧化活性的蛋白肽的酶解条件。结果表明,在中性蛋白酶的加酶量为15 mg/g、温度45℃、底物浓度5%、酶解3 h后,得到的酶解液对DPPH自由基清除率能达到85.46%。利用中性蛋白酶对低值鱼进行酶解可得到具有DPPH自由由基清除能力的活性肽。This study used neutral protease to hydrolyze protein of low-value little fish. The enzymatic hydrolysis process parameter was studied by using the index of enzymatic hydrolysis product of DPPH free radical clearance rate. The digestion process parameters was optimized through orthogonal test, It concluded that the strongest antioxidant activity of enzyme solution process parameters as follows： the adding amount was 15 mg/g, the hydrolysis temperature was 45 ℃, the substrate concentration was 5%, and the hydrolysis time was 3 h, under the technological conditions of DPPH free radical clearance rate could reach 85.46%.

关 键 词：DPPH自由基 抗氧化 低值鱼 中性蛋白酶 

分 类 号：TS254.9[轻工技术与工程—水产品加工及贮藏工程]