超高效液相色谱法同时测定饮料中14种食品添加剂  

Simultaneous determination of 14 kinds of food additives in beverage by ultra performance liquid chromatography

作　　者：牟霄 张璐[2] 张崇淼[2] 郭建博 朱小红 宋莉 马月 MOU Xiao ZHANG Lu ZHANG Chong-Miao GUO Jian-Bo ZHU Xlao-Hong SONG Li MA Yue(Shaanxi Institute for Food and Drug Control, Xi＇an 710065, China School of Environmental and Municipal Engineering, Xi ＇an University of Architecture and Technology, Xi ＇an 710055, China)

机构地区：[1]陕西省食品药品检验所,西安710065 [2]西安建筑科技大学环境与市政工程学院,西安710055

出　　处：《食品安全质量检测学报》2017年第8期3177-3184,共8页Journal of Food Safety and Quality

基　　金：陕西省社会发展科技攻关项目(2015SF273)~~

摘　　要：目的建立一种应用超高效液相色谱法同时测定饮料中14种着色剂、甜味剂和防腐剂的分析方法。方法样品经ZOXBAX SB-C_(18)色谱柱分离,以甲醇和100mmol/L磷酸二氢铵溶液为流动相进行梯度洗脱,流速0.2mL/min,柱温35℃。采用二极管阵列检测器,在190~600nm波长下进行扫描,230nm和200nm波长下进行检测,外标法定量。结果在最佳色谱条件下,这14种食品添加剂可在20min内有效分离,标准曲线线性关系良好(r>0.996)。平均回收率为92.7%~97.1%,相对标准偏差为0.61%~2.54%。当取样量为5g,稀释体积为25mL时,检出限(limits of detection,LODs)为0.3~0.6mg/kg。结论建立的方法可实现对饮料中多种着色剂、甜味剂和防腐剂的同时检测,具有快速、准确、灵敏的特点。Objective To establish a method for simultaneous determination of 14 kinds of coloring agents, sweeteners and antiseptics in beverage by ultra performance liquid chromatography （UPLC）.Methods The samples were separated by Agilent ZORBAX SB-C18 column with mobile phase consisting of methanol and ammonium dihydrogen phosphate solution （100 mmol/L）. The gradient elution was at a flow rate of 0.2 mL/min and column temperature was 35℃. These food additives were detected by diode array detector in the wavelength range from 190 to 600 nm with detection wave length at 230 nm and 200 nm. The detection results were quantitated by an external standard method.Results Under the optimal conditions, 14 kinds of food additives could be effectively separated within 20 min. The standard curves had good linearities （r〉0.996）. The average recoveries were in the range of 92.7% to 97.1% and the relative standard deviations （RSDs） were 0.61%～2.54%. The limits of detection （LODs） of 14 kinds of food additives were 0.3～0.6 ng.Conclusion The established method is rapid, accurate and sensitive, which is suitable for simultaneously detection of various coloring agents, sweeteners and antiseptics in beverage.

关 键 词：超高效液相色谱法 饮料 着色剂 甜味剂 防腐剂 

分 类 号：O657.72[理学—分析化学] TS27[理学—化学]