不同黑茶多糖的组成分析及其抗氧化活性  

Composition and antioxidant activity of polysaccharides of different dark tea

作　　者：杨新河 黄明军[1,2] 马蔚 舒婷[1,2] 何慧[2] 吕帮玉 

机构地区：[1]湖北省植物功能成分利用工程技术研究中心,湖北孝感432000 [2]湖北工程学院生命科学技术学院,湖北孝感432000

出　　处：《食品工业科技》2017年第20期16-20,共5页Science and Technology of Food Industry

基　　金：国家自然科学基金面上项目(31370692);湖北省自然科学基金面上项目(2014CFB573)

摘　　要：通过水提醇沉和Sevage法脱蛋白质获得茯砖茶、青砖茶、六堡茶、康砖茶、普洱茶和千两茶6种黑茶样品茶多糖,采用化学方法测定它们各自的中性糖、蛋白质、糖醛酸和多酚含量,并通过ABTS、DPPH、FRAP和总还原力等四种体系评价它们的抗氧化活性。结果表明,本次选用的6种黑茶样品茶多糖的组成与抗氧化活性有所差异,其中中性糖、蛋白质、糖醛酸和多酚四种成分含量范围分别为502.49~632.87,45.45~104.84,183.44~229.16,14.94~100.83 mg/g,且以普洱茶多糖的中性糖、蛋白质和多酚含量最高,而千两茶多糖的糖醛酸含量最高,此外它们均具有抗氧化能力,且在四种评价体系中从高到低的顺序相同,依次为普洱茶、康砖茶、六堡茶、青砖茶、茯砖茶和千两茶。本文为深入开展黑茶多糖组成与抗氧化活性研究及其功能产品开发提供了基础。The polysaccharides from dark tea of Fuzhuan tea,Qingzhuan tea,Liubao tea,Kangzhuan tea,Pu-erh tea,and Qianliang tea were prepared after extraction with hot water,precipitation with ethanol and deproteinization with sevage regent.Then,the neutral sugar,uronic acid,protein and polyphenol contents of six kinds of dark tea polysaccharides were measured using chemical methods.Finally,their antioxidant activities were evaluated using the ABTS assay,DPPH assay,FRAP assay and total reducing power assay.The results showed that there were some differences of chemical composition and antioxidant activity between six kinds of dark tea polysaccharides,and the contents of neutral sugar,protein,uronic acid and tea polyphenol of dark tea polysaccharides respectively were in ranges from 502.49 to 632.87 mg/g,45.45 to 104.84 mg/g,183.44 to 229.16 mg/g,14.94 to 100.83 mg/g,and Pu-erh tea polysaccharide contained the highest carbohydrate,protein and polyphenol contents while Qianliang tea polysaccharide contained the highest uronic acid content,and the order of their antioxidant capacity from high to low was Pu-erh tea,Kangzhuan tea,Liubao tea,Qingzhuan tea,Fuzhuan tea and Qianliang tea in all the four evaluation system.The results provided the basis for further research on composition and antioxidant activity of dark tea polysaccharides as well as their functional product development.

关 键 词：黑茶 多糖 组成 抗氧化活性 

分 类 号：TS272[农业科学—茶叶生产加工]