基于Miseq法纳豆冻干粉对小鼠肠道菌群影响的研究  

Effect of Natto Lyophilized Powder on Intestinal Flora of Mice Based on Miseq Method

作　　者：黄占旺[1] 王素贞[1] 吴高峰[1] 吴少福[1] 黄永平[1] 陈佳妮[1] 

机构地区：[1]江西农业大学食品科学与工程学院江西省天然产物与功能食品重点实验室,南昌330045

出　　处：《中国粮油学报》2017年第11期26-33,共8页Journal of the Chinese Cereals and Oils Association

基　　金：国家自然科学基金(31160337)

摘　　要：抗生素近年来被滥用,它抑制或杀死微生物使得机体菌群失调。因此找出一种抗生素的安全有效替代物非常有必要,而益生菌是一种有效的替代物。本研究以纳豆冻干粉为原料,正常小鼠和肠道菌群失衡小鼠为动物试验模型,采用体内灌胃试验和Miseq方法研究纳豆冻干粉对小鼠肠道菌群的影响并进行生物信息分析。分析多样本间的肠道菌群多样性和单样本属水平上的分类情况;分析纳豆芽孢杆菌及益生菌、致病菌的菌数变化情况。预期的研究结果为揭示纳豆芽孢杆菌的肠道菌群调节机理提供科学依据,对人类健康和传统发酵食品微生物的开发具有十分重要的意义。Antibiotics was abused in recent years, and it inhibits or kills microbes, which made the body dysbac- teriosis. Therefore, it is very necessary to look for a safe and effective antibiotic replacement, and probiotics is an effective alternate. Natto lyophilized powder was the raw material and the normal mice and low intestinal flora in mice was used as animal testing model. In vivo gavage experiment and Miseq method were used to study the effect of natto lyophilized powder on mice intestinal flora. Further the biological information of mice was analyzed. The diversity of intestinal flora among multiple samples and the classification of the single sample＇s level were analyzed. Ultimately, the changes in the number of bacteria in Bacillus natto and probiotics and pathogens were analyzed. The expected results provided the scientific basis of revealing the regulating mechanism of bacillus natto on the intestinal flora, and had a very vital significance for human health and the development of the traditional fermented food microorganism.

关 键 词：纳豆冻干粉 肠道菌群 Miseq法 多样性 

分 类 号：R151[医药卫生—营养与食品卫生学]