高效离子色谱法检测婴幼儿配方奶粉中的功能性低聚糖  

Determination of Functional Oligosaccharides in Infant Formula Milk Powder by High Performance Ion Chromatography

作　　者：朱伟[1] 宁啸骏[1] 杜茹芸 

机构地区：[1]上海市质量监督检验技术研究院,上海200233

出　　处：《食品科学》2017年第24期224-228,共5页Food Science

基　　金：国家质量监督检验检疫总局科技计划项目(2014QK149)

摘　　要：建立一种同时测定蔗果三糖、蔗果四糖、蔗果五糖、蔗果六糖、蔗果七糖、棉子糖、水苏糖、毛蕊花糖的高效离子色谱检测技术,采用CarboPac PA20色谱柱分离,以氢氧化钠-乙酸钠为流动相,梯度洗脱,使用脉冲安培检测器进行检测。结果表明,在本实验条件下8种功能性低聚糖得到了很好分离,回收率为80.0%~103%,相对标准偏差小于3%(n=6)。该方法能快速、准确、可靠地检测婴幼儿配方奶粉中功能性低聚糖的含量。A method was established using high performance ion chromatography and pulsed amperometric detection for simultaneous analysis of 1-kestose（GF2）,nystose（GF3）,1 F-fructofuranosyl nystose（GF4）,GF5,GF6,raffinose,stachyose and verbascose in infant formula milk powder.The chromatographic separation was achieved on a CarboPac PA20 column using a mobile phase composed of a mixture of sodium hydroxide and sodium acetate by gradient elution.Under the conditions optimized in this study,all the analytes were well separated,and the recoveries were 80.0%–103% with a relative standard deviation（RSD） less than 3%（n=6）.The method was rapid,accurate,reliable,and thus suitable for the determination of functional oligosaccharides in infant formula milk powder.

关 键 词：配方奶粉 功能性低聚糖 高效离子色谱 脉冲安培检测器 

分 类 号：O657.7[理学—分析化学]