高产β-葡萄糖苷酶菌株的筛选及产酶条件优化  

Screening of β-Glucosidase-highly-producing Strains and Optimization of Their Fermentation Conditions

作　　者：姚瑶[1] 霍元鹏 周伟 叶佳颖[1] 褚梦真 朱森林[2] 蒋红英[2] 

机构地区：[1]浙江师范大学地理与环境科学学院,浙江金华321004 [2]金华职业技术学院农业与生物工程学院,浙江金华321007

出　　处：《江西农业学报》2018年第3期97-101,共5页Acta Agriculturae Jiangxi

基　　金：金华市科技计划项目(2015-2-010)

摘　　要：通过七叶苷平板法从腐殖质土壤中筛选得到高产β-葡萄糖苷酶的2个菌株(H_2和H_5),并利用单因素法对这2个菌株的产酶条件进行了优化。经形态学特征初步鉴定,菌株H_2和H_5均为黑曲霉(Aspergillus niger)。H_2的最优发酵条件为:以蔗糖为碳源,以硫酸铵+尿素(1∶1)为氮源,培养5 d,培养温度28℃,初始pH 4.0。H_5的最优发酵条件为:以红糖为碳源,以硫酸铵+硝酸铵(1∶1)为氮源,培养5 d,培养温度28℃,初始pH 4.5。在优化的各因素中,以培养温度对菌株H_2和H_5所产β-葡萄糖苷酶活力的影响最大。Two β-glucosidase-highly-producing strains H2 and H5 were isolated and screened out from humus soil by adop？ting aesculin plate method, and their fermentation conditions for the high-efficient production of β-glucosidase were optimizedthrough single-factor experiments. Both H2 and H5 were preliminarily identified as Aspergillus niger through morphological observa？tion. The optimum fermentation conditions for H2 were obtained as follows： using sucrose as carbon source, using ammonium sul？fate plus urea （1 ∶1） as nitrogen source, culture for 5 days at 28 ℃, initial pH-value 4.0. The optimum fermentation conditionsfor H5 were： using brown sugar as carbon source, using ammonium sulfate plus ammonium nitrate （1 ∶1） as nitrogen source, cul？ture for 5 days at 28 ℃, initial pH-value 4.5. Among the optimized various factors, culture temperature had the greatest influenceon the activity of β-glucosidase produced by the above 2 strains.

关 键 词：Β-葡萄糖苷酶 菌株筛选 发酵条件 优化 

分 类 号：TQ925[轻工技术与工程—发酵工程]