母乳挥发性成分固相微萃取-气质联用检测条件的优化及测定  

Optimization of analysis conditions of volatile compounds in human breast milk by SPME-GC-MS

作　　者：高雅慧[1] 李子杰[2] GAO Ya-hui1 ,LI Zi-jie2(1.School of Food Science and Technology,Jiangnan University,Wuxi 214122, China; 2. Sehool of Biotechnology, Jiangnan University, Wuxi 214122, Chin)

机构地区：[1]江南大学食品学院,江苏无锡214122 [2]江南大学生物工程学院,江苏无锡214122

出　　处：《食品工业科技》2018年第6期199-203,共5页Science and Technology of Food Industry

基　　金：国家自然科学基金(21302069)

摘　　要：采用固相微萃取-气质联用分析母乳挥发性成分,并对其检测条件进行优化。通过单因素实验,最终确定了母乳挥发性成分较优的检测条件为使用 DVB/PDMS 萃取头、加入1.5 g NaCl的5 mL样品在60℃萃取30 min、解析5min。在此检测条件下分析母乳样品,得到34种挥发性成分,其中烯烃类14种、酸类5种、酯类8种、芳香烃类4种、酮类3种,将为改善配方奶的风味提供一定的理论依据。The combined technology of solid phase microextraction （ SPME ） and gas chromatography - mass spectrometry （GC-MS） was used to analyze the volatile compounds from human breast milk and the detection conditions were optimized by single factor experiments.The optimal conditions were achieved when 5 mL of sample containing 1.5 gram of NaC1 was extracted at 60 ℃ for 30 rain using DVB/PDMS extraction fiber with a resolution time of 5 min.Under optimal conditions, thirty- four volatile compounds including alkenes （ fourteen types ）, acids （ five types ） , esters （ eight types ）, aromatic hydrocarbons （ four types） and ketones（ three types）were identified. This study could provide theoretical basis for the improvement of flavor of formula milk.

关 键 词：固相微萃取 气质联用 母乳 挥发性成分 条件优化 

分 类 号：TS201.2[轻工技术与工程—食品科学]